Evidence map›Paper›PMID 30415131›Full record

ArticleVirology2019

Core gene insertion in hepatitis B virus genotype G functions at both the encoded amino acid sequence and RNA structure levels to stimulate core protein expression.

Jing Zhang, Li Zong, Yongxiang Wang, Cheng Li, Chaoyang Chen, Yumei Wen, Jisu Li, Shuping Tong

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.5field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 6 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Jing ZhangKey Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.
Li ZongKey Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.
Yongxiang WangKey Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.
Cheng LiKey Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.
Chaoyang ChenKey Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.
Yumei WenKey Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.
Jisu LiLiver Research Center, Rhode Island Hospital, The Alpert Warren School of Medicine, Brown University, Providence, RI, USA.
Shuping TongKey Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China; Liver Research Center, Rhode Island Hospital, The Alpert Warren School of Medicine, Brown University, Providence, RI, USA. Electronic address: shuping_tong_md@brown.edu.
Shanghai Medical College of Fudan University · CNBrown University · US

Funding

Hepatitis B virus vaccine escape mutantsR01AI116639 · NIAID · RHODE ISLAND HOSPITAL · PI TONG, SHUPING · 2015 to 2019
$1.8M
Control of HBV replication at the step of core and P protein translationR21AI107618 · NIAID · RHODE ISLAND HOSPITAL · PI LI, JISU · 2013 to 2014
$423k
NIAID NIH HHS R01 AI116639NIAID NIH HHS R21 AI107618
6 · The paper itself

Abstract

Hepatitis B virus genotype G possesses a 36-nucleotide (nt) insertion at the 5' end of core gene, adding 12 residues to core protein. The insertion markedly increased core protein level irrespective of viral genotype, with the effect reproducible using CMV-core gene construct. Here we used such expression constructs and transient transfection experiments in Huh7 cells to identify the structural bases. The insertion is predicted to create a stem-loop structure 14nt downstream of core gene AUG. A + 1 or + 2 frameshift into the 36nt mitigated enhancement of core protein level. Point mutations to disrupt or restore the stem-loop had opposite effects on core protein expression. Shifting the translation initiation site downstream or further upstream of the stem-loop rendered it inhibitory or no longer stimulatory of core protein expression. Therefore, both the reading frame and a properly positioned stem-loop structure contribute to marked increase in core protein expression by the 36-nt insertion.

Indexed as

Nucleic Acid ConformationAmino Acid SequenceCodon, InitiatorGene Expression Regulation, ViralGenotypeHepatitis B virusHumansInverted Repeat SequencesMutationOpen Reading FramesProtein BiosynthesisRNA, ViralViral Core ProteinsVirionVirus ReplicationCodon, InitiatorRNA, ViralViral Core ProteinsCore proteinGenotypeHepatitis B virusOpen reading frameRNA secondary structureTranslational control

Identifiers

PMID30415131
PMCPMC6283280
OpenAlexW2900236861

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.