Evidence map›Paper›PMID 30414933›Full record

ArticleBiochimica et biophysica acta. Bioenergetics2019

Engineering of B800 bacteriochlorophyll binding site specificity in the Rhodobacter sphaeroides LH2 antenna.

David J K Swainsbury, Kaitlyn M Faries, Dariusz M Niedzwiedzki, Elizabeth C Martin, Adam J Flinders, Daniel P Canniffe, Gaozhong Shen, Donald A Bryant, Christine Kirmaier, Dewey Holten and 1 more

Open access · hybridAbstract read
In one paragraph

Article in Biochimica et biophysica acta. Bioenergetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
2.1field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 51 citations in OpenAlex.

  1. Review
  2. Spectral changes of light-harvesting complex 2 lacking B800 bacteriochlorophyll a under neutral pH conditions.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2024
    Article
  3. Article
  4. Article
  5. ChlorophyllBiology · 2023
    Article
  6. Article
  7. Cryo-EM Structure of theBiochemistry · 2021
    Article
  8. Article
  9. Article
  10. Effects of low-molecular-weight polyols on the hydration status of the light-harvesting complex 2 from Rhodobacter sphaeroides 2.4.1.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2021
    Article
  11. Review
  12. Review
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

David J K SwainsburyDepartment of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK.
Kaitlyn M FariesDepartment of Chemistry, Washington University, St. Louis, MO 63130-4889, USA.
Dariusz M NiedzwiedzkiDepartment of Chemistry, Washington University, St. Louis, MO 63130-4889, USA.
Elizabeth C MartinDepartment of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK.
Adam J FlindersDepartment of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK.
Daniel P CanniffeDepartment of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK; Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
Gaozhong ShenDepartment of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
Donald A BryantDepartment of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
Christine KirmaierDepartment of Chemistry, Washington University, St. Louis, MO 63130-4889, USA.
Dewey HoltenDepartment of Chemistry, Washington University, St. Louis, MO 63130-4889, USA.
C Neil HunterDepartment of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK. Electronic address: c.n.hunter@sheffield.ac.uk.
University of Sheffield · GBWashington University in St. Louis · USPennsylvania State University · US

Funding

Biotechnology and Biological Sciences Research Council BB/M000265/1
6 · The paper itself

Abstract

The light-harvesting 2 complex (LH2) of the purple phototrophic bacterium Rhodobacter sphaeroides is a highly efficient, light-harvesting antenna that allows growth under a wide-range of light intensities. In order to expand the spectral range of this antenna complex, we first used a series of competition assays to measure the capacity of the non-native pigments 3-acetyl chlorophyll (Chl) a, Chl d, Chl f or bacteriochlorophyll (BChl) b to replace native BChl a in the B800 binding site of LH2. We then adjusted the B800 site and systematically assessed the binding of non-native pigments. We find that Arg

Indexed as

Protein EngineeringBacteriochlorophyll ABacteriochlorophyllsBinding SitesLight-Harvesting Protein ComplexesProtein BindingRhodobacter sphaeroidesSpectrometry, FluorescenceBacteriochlorophyll ABacteriochlorophyllsLight-Harvesting Protein ComplexesBacteriochlorophyllChlorophyllLH2Ligand bindingLight harvestingProtein engineeringRhodobacter sphaeroides

Identifiers

PMID30414933
PMCPMC6358721
OpenAlexW2899788921

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.