ArticleNature communications2018
Crystallographic and spectroscopic assignment of the proton transfer pathway in [FeFe]-hydrogenases.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Redox regulation in OJournal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2026Article
- Proton Transport as a Missing Design Variable in Immobilized Enzyme Catalysis.Chembiochem : a European journal of chemical biology · 2026Article
- Electrochemical characterization of C. pasteurianum hydrogenase II and three site-directed variants: catalysis and inhibition.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2026Article
- A Low-Potential π‑Extended Viologen Electron Donor Results in Increased HACS organic & inorganic Au · 2026Article
- Probing the Role of Accessory Domains in Oxygen Stability of [FeFe]-Hydrogenases.Journal of the American Chemical Society · 2026Article
- QM/MM Modeling of the Electronic Structure and Properties of the Fe-S Clusters inInorganic chemistry · 2026Article
- Probing the Role of Accessory FeS Clusters in Putative Sensory Group D [FeFe] Hydrogenase: Influence of the C-Terminal [4Fe-4S] Cluster on the Reactivity ofBiochemistry · 2026Article
- [FeFe] hydrogenases with modified azadithiolate bridgeheads serve as unidirectional hydrogen oxidation catalysts.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2026Article
- Article
- Reconsidering the Enzyme Kinetics of [FeFe]-Hydrogenases: Improved Turnover Rates and New Insights into pH and Potential Dependence with Eu(II)-Based Solution Assays.Analytical chemistry · 2025Article
- H-cluster Intermediates and Catalytic Properties ofBiochemistry · 2025Article
- Computational Modeling and Experimental Approaches for Understanding the Mechanisms of [FeFe]-Hydrogenase.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Distinct Valence States of the [4Fe4S] Cluster Revealed in the Hydrogenase CrHydA1.Angewandte Chemie (International ed. in English) · 2025Article
- Probing the Influence of the Protein Scaffold on H-Cluster Reactivity via Gain-of-Function Studies─Improved HJournal of the American Chemical Society · 2025Article
- Structural determinants of oxygen resistance and ZnProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Outer-sphere effects on the OChemical science · 2024Review
- Predicting the Structure of Enzymes with Metal Cofactors: The Example of [FeFe] Hydrogenases.International journal of molecular sciences · 2024Article
- Kinetic Modeling of the Reversible or Irreversible Electrochemical Responses of FeFe-Hydrogenases.Journal of the American Chemical Society · 2024Article
- Probing Substrate Transport Effects on Enzymatic Hydrogen Catalysis: An Alternative Proton Transfer Pathway in Putatively Sensory [FeFe] Hydrogenase.ACS catalysis · 2023Article
- A personal account on 25 years of scientific literature on [FeFe]-hydrogenase.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2023Review
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The unmatched catalytic turnover rates of [FeFe]-hydrogenases require an exceptionally efficient proton-transfer (PT) pathway to shuttle protons as substrates or products between bulk water and catalytic center. For clostridial [FeFe]-hydrogenase CpI such a pathway has been proposed and analyzed, but mainly on a theoretical basis. Here, eleven enzyme variants of two different [FeFe]-hydrogenases (CpI and HydA1) with substitutions in the presumptive PT-pathway are examined kinetically, spectroscopically, and crystallographically to provide solid experimental proof for its role in hydrogen-turnover. Targeting key residues of the PT-pathway by site directed mutagenesis significantly alters the pH-activity profile of these variants and in presence of H
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