ArticleNature communications2018
High-resolution ultramicroscopy of the developing and adult nervous system in optically cleared Drosophila melanogaster.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 73 citations in OpenAlex.
- Accelerated immunostaining of thick biological tissues using bidirectional electric fields with reversible deformation.Biomedical optics express · 2026Article
- activeDISCO enables large-scale exploration of human brain anatomy by light sheet microscopy.Communications biology · 2026Article
- Isotropic, aberration-corrected light sheet microscopy for rapid high-resolution imaging of cleared tissue.Nature biotechnology · 2025Article
- A multiwell plate approach to increase the sample throughput during tissue clearing.Nature protocols · 2025Review
- Whole organism and tissue-specific analysis of pexophagy inOpen biology · 2025Article
- Promising tools into oxidative stress: A review of non-rodent model organisms.Redox biology · 2024Review
- Improved whole-mount immunofluorescence protocol for consistent and robust labeling of adult Drosophila melanogaster adipose tissue.Biology open · 2024Article
- Real-time study of spatio-temporal dynamics (4D) of physiological activities in alive biological specimens with different FOVs and resolutions simultaneously.Scientific reports · 2024Article
- Akaluc/AkaLumine bioluminescence system enables highly sensitive, non-invasive and temporal monitoring of gene expression in Drosophila.Communications biology · 2023Article
- Insights into human kidney function from the study of Drosophila.Pediatric nephrology (Berlin, Germany) · 2023Review
- An optofluidic platform for interrogating chemosensory behavior and brainwide neural representation in larval zebrafish.Nature communications · 2023Article
- MAX: a simple, affordable, and rapid tissue clearing reagent for 3D imaging of wide variety of biological specimens.Scientific reports · 2022Article
- Oxidative stress mediates end-organ damage in a novel model of acetaminophen-toxicity in Drosophila.Scientific reports · 2022Article
- A hypothalamic dopamine locus for psychostimulant-induced hyperlocomotion in mice.Nature communications · 2022Article
- Light-sheet mesoscopy with the Mesolens provides fast sub-cellular resolution imaging throughout large tissue volumes.iScience · 2022Article
- Review
- Autofluorescent Biomolecules in Diptera: From Structure to Metabolism and Behavior.Molecules (Basel, Switzerland) · 2022Review
- FlyClear: A Tissue-Clearing Technique for High-Resolution Microscopy of Drosophila.Methods in molecular biology (Clifton, N.J.) · 2022Article
- NuMorph: Tools for cortical cellular phenotyping in tissue-cleared whole-brain images.Cell reports · 2021Article
- Tissue clearing and 3D imaging in developmental biology.Development (Cambridge, England) · 2021Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
The fruit fly, Drosophila melanogaster, is an important experimental model to address central questions in neuroscience at an organismic level. However, imaging of neural circuits in intact fruit flies is limited due to structural properties of the cuticle. Here we present a novel approach combining tissue clearing, ultramicroscopy, and data analysis that enables the visualisation of neuronal networks with single-cell resolution from the larval stage up to the adult Drosophila. FlyClear, the signal preserving clearing technique we developed, stabilises tissue integrity and fluorescence signal intensity for over a month and efficiently removes the overall pigmentation. An aspheric ultramicroscope set-up utilising an improved light-sheet generator allows us to visualise long-range connections of peripheral sensory and central neurons in the visual and olfactory system. High-resolution 3D reconstructions with isotropic resolution from entire GFP-expressing flies are obtained by applying image fusion from orthogonal directions. This methodological integration of novel chemical, optical, and computational techniques allows a major advance in the analysis of global neural circuit organisation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.