ArticleDevelopmental biology2019
I-SceI Meganuclease-mediated transgenesis in the acorn worm, Saccoglossus kowalevskii.
Article in Developmental biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 16 citations in OpenAlex.
- Deep homology of aScience advances · 2025Article
- Hemichordate cis-regulatory genomics and the gene expression dynamics of deuterostomes.Nature ecology & evolution · 2024Article
- Cephalopod-omics: Emerging Fields and Technologies in Cephalopod Biology.Integrative and comparative biology · 2023Article
- Molecular characterization of nervous system organization in the hemichordate acorn worm Saccoglossus kowalevskii.PLoS biology · 2023Article
- Transgenesis in the acoel worm Hofstenia miamia.Developmental cell · 2021Article
- Beyond Adult Stem Cells: Dedifferentiation as a Unifying Mechanism Underlying Regeneration in Invertebrate Deuterostomes.Frontiers in cell and developmental biology · 2020Review
- An atlas of anterior hox gene expression in the embryonic sea lamprey head: Hox-code evolution in vertebrates.Developmental biology · 2019Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Hemichordates are a phylum of marine invertebrate deuterostomes that are closely related to chordates, and represent one of the most promising models to provide insights into early deuterostome evolution. The genome of the hemichordate, Saccoglossus kowalevskii, reveals an extensive set of non-coding elements conserved across all three deuterostome phyla. Functional characterization and cross-phyla comparisons of these putative regulatory elements will enable a better understanding of enhancer evolution, and subsequently how changes in gene regulation give rise to morphological innovation. Here, we describe an efficient method of transgenesis for the characterization of non-coding elements in S. kowalevskii. We first test the capacity of an I-SceI transgenesis system to drive ubiquitous or regionalized gene expression, and to label specific cell types. Finally, we identified a minimal promoter that can be used to test the capacity of putative enhancers in S. kowalevskii. This work demonstrates that this I-SceI transgenesis technique, when coupled with an understanding of chromatin accessibility, can be a powerful tool for studying how evolutionary changes in gene regulatory mechanisms contributed to the diversification of body plans in deuterostomes.
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