ArticleBioconjugate chemistry2018
Control of Probe Density at DNA Biosensor Surfaces Using Poly(l-lysine) with Appended Reactive Groups.
Article in Bioconjugate chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- Practical Applications of 2D Material FET Biosensors: Functionalization Strategies and Detection Performance.Biosensors · 2026Review
- Automated Generation of Supported Lipid Bilayer Arrays with Controlled Receptor Densities in Well Plates.ACS applied materials & interfaces · 2026Article
- Material-Selective Deposition of Reactive Group-Functionalized Poly‑l‑lysine for DNA Sensing at Optical Waveguides.ACS omega · 2025Article
- Identification of novel human IgE-binding peptides from a phage display library for total IgE detection.Scientific reports · 2025Article
- Covalent organic frameworks in cancer theranostics: advancing biomarker detection and tumor-targeted therapy.Archives of pharmacal research · 2025Review
- Analyzing Binding Specificity in a Microparticle-Based DNA Displacement Assay Using Multiharmonic QCM-D.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Detection of miR-155 Using Peptide Nucleic Acid at Physiological-like Conditions by Surface Plasmon Resonance and Bio-Field Effect Transistor.Biosensors · 2024Article
- Achieving high hybridization density at DNA biosensor surfaces using branched spacer and click chemistry.RSC advances · 2023Article
- Interface Engineering of "Clickable" Organic Electrochemical Transistors toward Biosensing Devices.ACS applied materials & interfaces · 2023Article
- Optimization of Functional Group Concentration of N, N-Dimethylacrylamide-based Polymeric Coatings and Probe Immobilization for DNA and Protein Microarray Applications.Micromachines · 2023Article
- Au Nanoparticle-Based Amplified DNA Detection on Poly-l-lysine Monolayer-Functionalized Electrodes.Nanomaterials (Basel, Switzerland) · 2022Article
- Poly(α-l-lysine)-based nanomaterials for versatile biomedical applications: Current advances and perspectives.Bioactive materials · 2021Review
- Detection of Tumor DNA in Human Plasma with a Functional PLL-Based Surface Layer and Plasmonic Biosensing.ACS sensors · 2021Article
- Biosensing strategies for the electrochemical detection of viruses and viral diseases - A review.Analytica chimica acta · 2021Review
- Conformational Changes of Immobilized Polythymine due to External Stressors Studied with Temperature-Controlled Electrochemical Microdevices.Langmuir : the ACS journal of surfaces and colloids · 2021Article
- Recent Progress in Intelligent Wearable Sensors for Health Monitoring and Wound Healing Based on Biofluids.Frontiers in bioengineering and biotechnology · 2021Review
- Enhancement of Probe Density in DNA Sensing by Tuning the Exponential Growth Regime of Polyelectrolyte Multilayers.Chemistry of materials : a publication of the American Chemical Society · 2020Article
- Increasing the Sensitivity of Electrochemical DNA Detection by a Micropillar-Structured Biosensing Surface.Langmuir : the ACS journal of surfaces and colloids · 2020Article
- Surface Modification with Control over Ligand Density for the Study of Multivalent Biological Systems.ChemistryOpen · 2020Review
- "Plug-n-Play" Polymer Substrates: Surface Patterning with Reactive-Group-Appended Poly-l-lysine for Biomolecule Adhesion.ACS applied polymer materials · 2019Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biosensors and materials for biomedical applications generally require chemical functionalization to bestow their surfaces with desired properties, such as specific molecular recognition and antifouling properties. The use of modified poly(l-lysine) (PLL) polymers with appended oligo(ethylene glycol) (OEG) and thiol-reactive maleimide (Mal) moieties (PLL-OEG-Mal) offers control over the presentation of functional groups. These reactive groups can readily be conjugated to, for example, probes for DNA detection. Here we demonstrate the reliable conjugation of thiol-functionalized peptide nucleic acid (PNA) probes onto predeposited layers of PLL-OEG-Mal and the control over their surface density in the preceding synthetic step of the PLL modification with Mal groups. By monitoring the quartz crystal microbalance (QCM) frequency shifts of the binding of complementary DNA versus the density of Mal moieties grafted to the PLL, a linear relationship between probe density and PLL grafting density was found. Cyclic voltammetry experiments using Methylene Blue-functionalized DNA were performed to establish the absolute probe density values at the biosensor surfaces. These data provided a density of 1.2 × 10
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