ArticleFrontiers in immunology2018
Choline Regulates the Function of Bovine Immune Cells and Alters the mRNA Abundance of Enzymes and Receptors Involved in Its Metabolism
Article in Frontiers in immunology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- Supplementation of rumen-protected choline during warm summer days reduces body surface temperature and improves dry matter intake of Holstein dairy cows.JDS communications · 2026Article
- Metabolic and Lactation Effects of Rumen-Protected Choline Supplementation in Peripartum Dairy Cows and Its Effects on Calf Growth Until Weaning.Metabolites · 2026Article
- Inflammation in the Transition Period of Dairy Cows: New Paradigms.Veterinary sciences · 2025Review
- Effects of repeated 14-day oral toxicity of fermentedOpen veterinary journal · 2025Article
- Effects of Dietary Rumen-Protected Choline on Stress Alleviation, Antioxidant Modulation, and Haematological, Immunological, and Offspring Performance in Transitioning Goats.Veterinary medicine and science · 2025Article
- The In Vitro Effects of Choline on Non-Esterified Fatty Acid-Treated Bovine Peripheral Blood Leukocytes.Animals : an open access journal from MDPI · 2025Article
- Effects of dietary rumen-protected choline supplementation on genome-wide DNA methylation in mammary tissue from postpartum dairy cows.JDS communications · 2025Article
- Choline in immunity: a key regulator of immune cell activation and function.Frontiers in immunology · 2025Review
- Effects of fermentedOpen veterinary journal · 2024Article
- Betaine and L-Carnitine Synergistically Influence the Metabolome and Immune Response in Dogs.Animals : an open access journal from MDPI · 2024Article
- Genetically predicted metabolites mediate the causal associations between autoimmune thyroiditis and immune cells.Frontiers in endocrinology · 2024Article
- The Effects of Rumen-Protected Choline and Rumen-Protected Nicotinamide on Liver Transcriptomics in Periparturient Dairy Cows.Metabolites · 2023Article
- Exploring the Immune-Boosting Functions of Vitamins and Minerals as Nutritional Food Bioactive Compounds: A Comprehensive Review.Molecules (Basel, Switzerland) · 2022Review
- Overview of the effect of rumen-protected limiting amino acids (methionine and lysine) and choline on the immunity, antioxidative, and inflammatory status of periparturient ruminants.Frontiers in immunology · 2022Review
- Role of Choline in Ocular Diseases.International journal of molecular sciences · 2021Review
- CpG-ODN induced antimicrobial immunity in neonatal chicks involves a substantial shift in serum metabolic profiles.Scientific reports · 2021Article
- Nuclear magnetic resonance (NMR)-based metabolome profile evaluation in dairy cows with and without displaced abomasum.The veterinary quarterly · 2020Article
- LC-QTOF-MS andPathogens (Basel, Switzerland) · 2020Article
- QuantitativeMetabolites · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dietary choline can impact systemic immunity, but it remains unclear whether this is primarily via direct impacts on immune cells or secondary effects of altered metabolic function. To determine whether increased choline concentrations (3.2, 8.2, 13.2 μM) in cell culture alter the function of bovine innate and adaptive immune cells, we isolated cells from dairy cows in early and mid-lactation as models of immuno-compromised and competent cells, respectively. Phagocytic and killing capacity of isolated neutrophils were linearly diminished with increasing doses of choline. In contrast, lymphocyte proliferation was linearly enhanced with increasing doses of choline. Furthermore, increasing doses of choline increased the mRNA abundance of genes involved in the synthesis of choline products (betaine, phosphatidylcholine, and acetylcholine) as well as muscarinic and nicotinic acetylcholine receptors in a quadratic and linear fashion for neutrophils and monocytes, respectively. Phagocytic and killing capacity of neutrophils and proliferation of lymphocytes were not affected by stage of lactation or its interaction with choline or LPS. In neutrophils from early lactation cows, choline linearly increased the mRNA abundance of muscarinic and nicotinic cholinergic receptors, whereas choline-supplemented monocytes from mid-lactation cows linearly increased the mRNA abundance of several genes coding for choline metabolism enzymes. These data demonstrate that choline regulates the inflammatory response of immune cells and suggest that the mechanism may involve one or more of its metabolic products.
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