Evidence map›Paper›PMID 30409987›Full record

ArticleScientific reports2018

Improving genetic transformation rates in honeybees.

M Otte, O Netschitailo, O Kaftanoglu, Y Wang, R E Page, M Beye

Abstract read
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Dual-line Genome-scale CRISPR Screening Enables Robust Target Gene Discovery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
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  12. EfficientFrontiers in genome editing · 2022
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

M OtteEvolutionary Genetics, Heinrich Heine University Düsseldorf, Düsseldorf, Germany. otte@hhu.de.
O NetschitailoEvolutionary Genetics, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
O KaftanogluSchool of Life Sciences, Arizona State University, Tempe, United States.
Y WangSchool of Life Sciences, Arizona State University, Tempe, United States.
R E PageSchool of Life Sciences, Arizona State University, Tempe, United States.
M BeyeEvolutionary Genetics, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Functional genetic studies in honeybees have been limited by transformation tools that lead to a high rate of transposon integration into the germline of the queens. A high transformation rate is required to reduce screening efforts because each treated queen needs to be maintained in a separate honeybee colony. Here, we report on further improvement of the transformation rate in honeybees by using a combination of different procedures. We employed a hyperactive transposase protein (hyPBase

Indexed as

AnimalsAnimals, Genetically ModifiedBeesFemaleGene Expression ProfilingGene Expression Regulation, DevelopmentalInjectionsInsect ProteinsMaleRNA, MessengerSf9 CellsTransformation, GeneticTransposasesInsect ProteinsRNA, MessengerTransposases

Identifiers

PMID30409987
PMCPMC6224437

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.