ArticleBMC gastroenterology2018
Evaluating gut microbiota profiles from archived fecal samples.
Article in BMC gastroenterology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 41 citations in OpenAlex.
- The CRCbiome study: a large prospective cohort study examining the role of lifestyle and the gut microbiome in colorectal cancer screening participants.BMC cancer · 2021Trial
- CRISPR-Cas immune repertoires as an ecological record of bacterial interactions with mobile genetic elements in the human gut.Gut microbes · 2026Article
- Fecal immunochemical tests from population-based colorectal cancer screening programs support prospective microbiome cohorts.British journal of cancer · 2026Article
- Fecal Sample Collection for Gut Microbiome Research in a Prospective Cohort: A Pilot Study within the Australian Breakthrough Cancer Study.Cancer research communications · 2026Article
- Comparative Analysis of Compound Probiotics, Seasonal Variation, and Age on Gut Microbial Composition and Function in Endangered Forest Musk Deer.Microorganisms · 2025Article
- Fecal occult blood affects intestinal microbial community structure in colorectal cancer.BMC microbiology · 2025Article
- Probiotic administration correlated with reduced diarrheal incidence and improved gut microbiota diversity in young goats.Frontiers in veterinary science · 2025Article
- Integrated omics analysis reveals the alteration of gut microbiota and fecal metabolites in Cervus elaphus kansuensis.Applied microbiology and biotechnology · 2024Article
- Species-level verification ofmSystems · 2024Article
- Exploring the gut DNA virome in fecal immunochemical test stool samples reveals associations with lifestyle in a large population-based study.Nature communications · 2024Article
- Profiling small RNAs in fecal immunochemical tests: is it possible?Molecular cancer · 2023Article
- Long-term follow-up of colorectal cancer screening attendees identifies differences inFrontiers in oncology · 2023Article
- Reusing a prepaid health plan's fecal immunochemical tests for microbiome associations with colorectal adenoma.Scientific reports · 2022Article
- Cryopreservation of stool samples altered the microbial viability quantitively and compositionally.Archives of microbiology · 2022Article
- Comparative analysis of gut microbial composition and potential functions in captive forest and alpine musk deer.Applied microbiology and biotechnology · 2022Article
- Comparison of Fecal Sample Collection Methods for Microbial Analysis Embedded within Colorectal Cancer Screening Programs.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2022Article
- Different living environments drive deterministic microbial community assemblages in the gut of Alpine musk deer (Frontiers in microbiology · 2022Article
- Characterization of gut microbiota dysbiosis of diarrheic adult yaks through 16S rRNA gene sequences.Frontiers in veterinary science · 2022Article
- Using fecal immunochemical tubes for the analysis of the gut microbiome has the potential to improve colorectal cancer screening.Scientific reports · 2021Article
- Dysbiosis in intestinal microbiome linked to fecal blood determined by direct hybridization.3 Biotech · 2020Article
Corrections and comments
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Authors and funding
6 authors at 6 institutions in 2 countries.
Funding
Abstract
backgroundAssociations between colorectal cancer and microbiota have been identified. Archived fecal samples might be valuable sample sources for investigating causality in carcinogenesis and biomarkers discovery due to the potential of performing longitudinal studies. However, the quality, quantity and stability of the gut microbiota in these fecal samples must be assessed prior to such studies. We evaluated i) cross-contamination during analysis for fecal blood and ii) evaporation in stored perforated fecal immunochemical tests (iFOBT) samples, iii) temperature stability as well as iv) comparison of the gut microbiota diversity and composition in archived, iFOBT and fresh fecal samples in order to assess feasibility of large scale microbiota studies.
methodsThe microbiota profiles were obtained by sequencing the V3-V4 region of 16S rDNA gene.
resultsThe iFOBT does not introduce any cross-sample contamination detectable by qPCR. Neither could we detect evaporation during freeze-thaw cycle of perforated iFOBT samples. Our results confirm room temperature stability of the gut microbiome. Diverse microbial profiles were achieved in 100% of fresh, 81% of long-term archived and 96% of iFOBT samples. Microbial diversity and composition were comparable between fresh and iFOBT samples, however, diversity differed significantly between long-term archived, fresh and iFOBT samples.
conclusionOur data showed that it is feasible to exploit archived fecal sample sets originally collected for testing of fecal blood. The advantages of using these sample sets for microbial biomarker discovery and longitudinal observational studies are the availability of high-quality diagnostic and follow-up data. However, care must be taken when microbiota are profiled in long-term archived fecal samples.
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