ArticleACS synthetic biology2018
Sigma Factor-Mediated Tuning of Bacterial Cell-Free Synthetic Genetic Oscillators.
Article in ACS synthetic biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Mixed positive and negative feedback loops drive diverse single-cell gene expression dynamics.Nature communications · 2026Article
- Controlling Multiphase Coacervate Wetting and Self-Organization by Interfacial Proteins.Journal of the American Chemical Society · 2025Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Review
- Regulatory Components for Bacterial Cell-Free Systems Engineering.ACS synthetic biology · 2024Review
- Building Synthetic Cells─From the Technology Infrastructure to Cellular Entities.ACS synthetic biology · 2024Review
- Plasmid Crosstalk in Cell-Free Expression Systems.ACS synthetic biology · 2023Article
- Noise Minimization in Cell-Free Gene Expression.ACS synthetic biology · 2023Article
- Mechanosensitive non-equilibrium supramolecular polymerization in closed chemical systems.Nature communications · 2023Article
- Network regulation meets substrate modification chemistry.Journal of the Royal Society, Interface · 2023Article
- What remains from living cells in bacterial lysate-based cell-free systems.Computational and structural biotechnology journal · 2023Review
- Complex dynamics in a synchronized cell-free genetic clock.Nature communications · 2022Article
- Memory, switches, and an OR-port through bistability in chemically fueled crystals.Nature communications · 2022Article
- DNA Input Classification by a Riboregulator-Based Cell-Free Perceptron.ACS synthetic biology · 2022Article
- Cell-Free Characterization of Coherent Feed-Forward Loop-Based Synthetic Genetic Circuits.ACS synthetic biology · 2021Article
- Optimising protein synthesis in cell-free systems, a review.Engineering biology · 2021Article
- Decentralizing Cell-Free RNA Sensing With the Use of Low-Cost Cell Extracts.Frontiers in bioengineering and biotechnology · 2021Article
- Effective Use of Linear DNA in Cell-Free Expression Systems.Frontiers in bioengineering and biotechnology · 2021Review
- Light-powered COScience (New York, N.Y.) · 2020Article
- Multiphase Complex Coacervate Droplets.Journal of the American Chemical Society · 2020Article
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7 authors.
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Abstract
Cell-free transcription-translation provides a simplified prototyping environment to rapidly design and study synthetic networks. Despite the presence of a well characterized toolbox of genetic elements, examples of genetic networks that exhibit complex temporal behavior are scarce. Here, we present a genetic oscillator implemented in an E. coli-based cell-free system under steady-state conditions using microfluidic flow reactors. The oscillator has an activator-repressor motif that utilizes the native transcriptional machinery of E. coli: the RNAP and its associated sigma factors. We optimized a kinetic model with experimental data using an evolutionary algorithm to quantify the key regulatory model parameters. The functional modulation of the RNAP was investigated by coupling two oscillators driven by competing sigma factors, allowing the modification of network properties by means of passive transcriptional regulation.
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