ArticlePloS one2018
Influence of warming and reanimation conditions on seminiferous tubule morphology, mitochondrial activity, and cell composition of vitrified testicular tissues in the domestic cat model.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Testicular cryopreservation: From technical aspects to practical applications.Histology and histopathology · 2025Review
- Evaluation of Different Cryoprotectant Combinations in Testicular Vitrification in Dogs.Reproduction in domestic animals = Zuchthygiene · 2025Article
- Solid Surface Vitrification Is Better than Slow Freezing for the Long-Term Preservation of Testicular Fragments from Prepubertal Collared Peccaries (Animals : an open access journal from MDPI · 2025Article
- Influence of Three Combinations of Cryoprotectants and Two Warming Temperatures on Cellular Morphology, Morphometry and Mitochondrial Activity of Vitrified Canine Testicles.Reproduction in domestic animals = Zuchthygiene · 2025Article
- Cryopreservation and culture strategies for testicular tissue and cells in small and large animals.Frontiers in veterinary science · 2025Review
- Review
- Effect of Different Vitrification Techniques on Viability and Apoptotic Index of Domestic Cat Testicular Tissue Cells.Animals : an open access journal from MDPI · 2023Article
- Testicular Tissue Vitrification: a Promising Strategy for Male Fertility Preservation.Reproductive sciences (Thousand Oaks, Calif.) · 2023Review
- Technologies for Vitrification Based Cryopreservation.Bioengineering (Basel, Switzerland) · 2023Review
- Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?Frontiers in endocrinology · 2023Review
- Canine and Feline Testicular Preservation.Animals : an open access journal from MDPI · 2022Review
- Feline microRNAome in ovary and testis: Exploration ofFrontiers in genetics · 2022Article
- Transcriptome dynamics in developing testes of domestic cats and impact of age on tissue resilience to cryopreservation.BMC genomics · 2021Article
- Strategies for cryopreservation of testicular cells and tissues in cancer and genetic diseases.Cell and tissue research · 2021Review
- Long-term Preservation of Testicular Tissue Integrity and Viability Using Vitrification in the Endangered Black-Footed Ferret (Animals : an open access journal from MDPI · 2020Article
- Three-dimensional culture of endometrial cells from domestic cats: A new in vitro platform for assessing plastic toxicity.PloS one · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding critical roles of warming and reanimation is critical to improve the survival of vitrified testicular tissue in domestic cats. The objective was to study structural and functional properties of testicular tissues from prepubertal domestic cats after standard vitrification followed by two warming protocols (directly at 37°C or with a 5-second pre-exposure to 50°C) and three reanimation time points (immediately, 24 h and 5 days post-warming). In Experiment 1, tissues were evaluated for histo-morphology and mitochondrial activity immediately or 24 h after warming protocols. In Experiment 2, cell viability, DNA fragmentation, and germ cell composition were assessed immediately, 24 h, or 5 days after optimal warming. Preservation of seminiferous tubule structure was better using warming at 50°C for five seconds, and survival of somatic as well as germinal cells was higher compared to direct warming at 37°C for one minute. Short term in vitro culture (for reanimation) also proved that cellular composition and functionality were better preserved when warmed for a short time at 50°C. Collective data showed that short warming at 50°C led to better quality of seminiferous tubule structure and cell composition after vitrification and short-term culture. In addition, data suggest clear directions to further understand and optimize testicular tissue survival after fertility preservation procedures.
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