ArticleThe Journal of cell biology1987
Reversible binding of actin to gelsolin and profilin in human platelet extracts.
Article in The Journal of cell biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed, 165 citations in OpenAlex.
- Small molecule intervention of actin-binding protein profilin1 reduces tumor angiogenesis in renal cell carcinoma.bioRxiv : the preprint server for biology · 2025Article
- Rho GTPase Signaling in Platelet Regulation and Implication for Antiplatelet Therapies.International journal of molecular sciences · 2023Review
- Articular Chondrocyte Phenotype Regulation through the Cytoskeleton and the Signaling Processes That Originate from or Converge on the Cytoskeleton: Towards a Novel Understanding of the Intersection between Actin Dynamics and Chondrogenic Function.International journal of molecular sciences · 2021Review
- The membrane-associated fraction of cyclase associate protein 1 translocates to the cytosol upon platelet stimulation.Scientific reports · 2018Article
- Actin polymerization in differentiated vascular smooth muscle cells requires vasodilator-stimulated phosphoprotein.American journal of physiology. Cell physiology · 2010Article
- Studies on the actin-binding protein HS1 in platelets.BMC cell biology · 2007Article
- Protease activated receptors in cardiovascular function and disease.Molecular and cellular biochemistry · 2004Article
- Regulation of the formation of osteoclastic actin rings by proline-rich tyrosine kinase 2 interacting with gelsolin.The Journal of cell biology · 2003Article
- Importance of free actin filament barbed ends for Arp2/3 complex function in platelets and fibroblasts.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Actin dynamics in platelets.International review of cytology · 2002Review
- VASP protects actin filaments from gelsolin: an in vitro study with implications for platelet actin reorganizations.Cell motility and the cytoskeleton · 2000Article
- Gelsolin and ADF/cofilin enhance the actin dynamics of motile cells.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Ca2+ regulation of gelsolin activity: binding and severing of F-actin.Biophysical journal · 1998Article
- Gelsolin modulates phospholipase C activity in vivo through phospholipid binding.The Journal of cell biology · 1997Article
- ADP-ribosylation of actin by Clostridium perfringens iota toxin and turkey erythrocyte ADP-ribosyltransferase A: effects on profilin-regulated nucleotide exchange and ATPase activity.Naunyn-Schmiedeberg's archives of pharmacology · 1996Article
- Coordinated regulation of platelet actin filament barbed ends by gelsolin and capping protein.The Journal of cell biology · 1996Article
- Phosphoinositides and phosphoinositide-utilizing enzymes in detergent-insoluble lipid domains.Molecular biology of the cell · 1996Article
- Actin filament barbed-end capping activity in neutrophil lysates: the role of capping protein-beta 2.Molecular biology of the cell · 1995Article
- Molecular cloning and characterization of profilin from tobacco (Nicotiana tabacum): increased profilin expression during pollen maturation.Plant molecular biology · 1995Article
- Activation of protein kinase C in rat basophilic leukemia cells stimulates increased production of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate: correlation with actin polymerization.Molecular biology of the cell · 1995Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
This paper documents the reversible appearance of high-affinity complexes of profilin and gelsolin with actin in extracts of platelets undergoing activation and actin assembly. Sepharose beads coupled to either monoclonal anti-gelsolin antibodies or to polyproline were used to extract gelsolin and profilin, respectively, from EGTA-containing platelet extracts and determine the proportion of these molecules bound to actin with sufficient affinity to withstand dilution (high-affinity complexes). Resting platelets (incubated for 30 min at 37 degrees C after gel filtration) contained nearly no high-affinity actin/gelsolin or actin/profilin complexes. Thrombin, within seconds, caused quantitative conversion of platelet profilin and gelsolin to high-affinity complexes with actin, but these complexes were not present 5 min after stimulation. The calcium-dependent actin filament-severing activity of platelet extracts, a function of free gelsolin, fell in concert with the formation of EGTA-stable actin/gelsolin complexes, and rose when the adsorption experiments indicated that free gelsolin was restored. The dissociation of high-affinity complexes was temporally correlated with the accumulation of actin in the Triton-insoluble cytoskeleton.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.