ArticleJournal of feline medicine and surgery2019
Diagnosis of non-effusive feline infectious peritonitis by reverse transcriptase quantitative PCR from mesenteric lymph node fine-needle aspirates.
Article in Journal of feline medicine and surgery, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 35 citations in OpenAlex.
- Central nervous system feline infectious peritonitis with a mass lesion in the fourth ventricle revealed by magnetic resonance imaging.The Journal of veterinary medical science · 2026Article
- Diagnosis of pyogranulomatous and neutrophilic lymphadenitis in 72 cats presenting to a referral hospital: with a focus on nine cats with steroid-responsive lymphadenitis.Journal of feline medicine and surgery · 2026Article
- Applying Supervised Machine Learning to Effusion Analysis for the Diagnosis of Feline Infectious Peritonitis.Bioengineering (Basel, Switzerland) · 2026Article
- Feline Infectious Peritonitis: A Challenging Diagnostic and Therapeutic Labyrinth.Animals : an open access journal from MDPI · 2026Review
- Rapid Clinical Resolution and Differential Diagnosis of a Neurological Case of Feline Infectious Peritonitis (FIP) Using GS-441524.Pathogens (Basel, Switzerland) · 2025Article
- A review of feline infectious peritonitis virus infection.Veterinary world · 2024Review
- Myocarditis in an FIP-Diseased Cat with FCoV M1058L Mutation: Clinical and Pathological Changes.Animals : an open access journal from MDPI · 2024Article
- Clinicopathological and Molecular Analysis of Aqueous Humor for the Diagnosis of Feline Infectious Peritonitis.Veterinary sciences · 2024Article
- Assessing the feasibility of applying machine learning to diagnosing non-effusive feline infectious peritonitis.Scientific reports · 2024Article
- GS-441524 and molnupiravir are similarly effective for the treatment of cats with feline infectious peritonitis.Frontiers in veterinary science · 2024Article
- Retrospective study and outcome of 307 cats with feline infectious peritonitis treated with legally sourced veterinary compounded preparations of remdesivir and GS-441524 (2020-2022).Journal of feline medicine and surgery · 2023Article
- Review
- Article
- Article
- An updated review of feline coronavirus: mind the two biotypes.Virus research · 2023Review
- 2022 AAFP/EveryCat Feline Infectious Peritonitis Diagnosis Guidelines.Journal of feline medicine and surgery · 2022Review
- Detection of Feline Coronavirus Variants in Cats without Feline Infectious Peritonitis.Viruses · 2022Article
- Successful management of feline pemphigus foliaceus with pentoxifylline and topical hydrocortisone aceponate.Veterinary medicine and science · 2022Article
- Article
- Molecular Detection of Feline Coronavirus Based on Recombinase Polymerase Amplification Assay.Pathogens (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
Abstract
objectivesThe aim of this study was to evaluate a feline coronavirus (FCoV) reverse transcriptase quantitative PCR (RT-qPCR) on fine-needle aspirates (FNAs) from mesenteric lymph nodes (MLNs) collected in sterile saline for the purpose of diagnosing non-effusive feline infectious peritonitis (FIP) in cats.
methodsFirst, the ability of the assay to detect viral RNA in MLN FNA preparations compared with MLN biopsy preparations was assessed in matched samples from eight cats. Second, a panel of MLN FNA samples was collected from a series of cats representing non-effusive FIP cases (n = 20), FCoV-seropositive individuals (n = 8) and FCoV-seronegative individuals (n = 18). Disease status of the animals was determined using a combination of gross pathology, histopathology and/or 'FIP profile', consisting of serology, clinical pathology and clinical signs.
resultsViral RNA was detected in 18/20 non-effusive FIP cases; it was not detected in two cases that presented with neurological FIP. Samples from 18 seronegative non-FIP control cats and 7/8 samples from seropositive non-FIP control cats contained no detectable viral RNA. Thus, as a method for diagnosing non-effusive FIP, MLN FNA RT-qPCR had an overall sensitivity of 90.0% and specificity of 96.1%. CONCLUSIONS AND RELEVANCE: In cases with a high index of suspicion of disease, RT-qPCR targeting FCoV in MLN FNA can provide important information to support the ante-mortem diagnosis of non-effusive FIP. Importantly, viral RNA can be reliably detected in MLN FNA samples in saline submitted via the national mail service. When applied in combination with biochemistry, haematology and serological tests in cases with a high index of suspicion of disease, the results of this assay may be used to support a diagnosis of non-effusive FIP.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.