ArticleAnalytical and bioanalytical chemistry2019
Comprehensive analysis of oxylipins in human plasma using reversed-phase liquid chromatography-triple quadrupole mass spectrometry with heatmap-assisted selection of transitions.
Article in Analytical and bioanalytical chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Trial
- Influence of 2 Weeks of Mango Ingestion on Inflammation Resolution after Vigorous Exercise.Nutrients · 2023Trial
- Blueberry intake elevates post-exercise anti-inflammatory oxylipins: a randomized trial.Scientific reports · 2023Trial
- Carbohydrate intake attenuates post-exercise plasma levels of cytochrome P450-generated oxylipins.PloS one · 2019Trial
- Total oxylipin concentrations in NIST SRM 1950 plasma compared to fresh and commercially obtained human plasma.Journal of lipid research · 2026Article
- Novel Insights into Systemic Hyaluronic Acid Therapy in Dogs with Osteoarthritis from an Exploratory Postmarketing Study: Clinical Improvements Linked to Biomarker Changes.Animals : an open access journal from MDPI · 2025Article
- Effect of Cannabidiol and Δ9-tetrahydrocannabinol on Anti-Inflammatory Lipid Mediator Synthesis in Humans.Cannabis and cannabinoid research · 2025Article
- 12/15-lipoxygenase mediates disturbed flow-induced endothelial dysfunction and atherosclerosis.Molecular medicine (Cambridge, Mass.) · 2025Article
- Deducing formation routes of oxylipins by quantitative multiple heart-cutting achiral-chiral 2D-LC-MS.Journal of lipid research · 2024Article
- Review
- Profiling of Oxylipins as Markers of Oxidative Stress in Biological Samples.Current protocols · 2024Article
- Beet supplementation mitigates post-exercise inflammation.Frontiers in nutrition · 2024Article
- Astaxanthin supplementation counters exercise-induced decreases in immune-related plasma proteins.Frontiers in nutrition · 2023Article
- Role of Oxylipins in the Inflammatory-Related Diseases NAFLD, Obesity, and Type 2 Diabetes.Metabolites · 2022Review
- Simultaneous profiling and quantification of 25 eicosanoids in human serum by ultrahigh-performance liquid chromatography coupled to tandem mass spectrometry.Analytical and bioanalytical chemistry · 2022Article
- Almond intake alters the acute plasma dihydroxy-octadecenoic acid (DiHOME) response to eccentric exercise.Frontiers in nutrition · 2022Article
- Quantification of Plasma Oxylipins Using Solid-Phase Extraction and Reversed-Phase Liquid Chromatography-Triple Quadrupole Mass Spectrometry.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Race-based and sex-based differences in bioactive lipid mediators after myocardial infarction.ESC heart failure · 2020Article
- Methods of the Analysis of Oxylipins in Biological Samples.Molecules (Basel, Switzerland) · 2020Review
- Blueberry and/or Banana Consumption Mitigate Arachidonic, Cytochrome P450 Oxylipin Generation During Recovery From 75-Km Cycling: A Randomized Trial.Frontiers in nutrition · 2020Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Oxylipins, a subclass of lipid mediators, are metabolites of various polyunsaturated fatty acids with crucial functions in regulation of systemic inflammation. Elucidation of their roles in pathological conditions requires accurate quantification of their levels in biological samples. We refined an ultra-performance liquid chromatography-multiple reaction monitoring-mass spectrometry (UPLC-MRM-MS)-based workflow for comprehensive and specific quantification of 131 endogenous oxylipins in human plasma, in which we optimized LC mobile phase additives, column, and gradient conditions. We employed heatmap-assisted strategy to identify unique transitions to improve the assay selectivity and optimized solid phase extraction procedures to achieve better analyte recovery. The method was validated according to FDA guidelines. Overall, 94.4% and 95.7% of analytes at tested concentrations were within acceptable accuracy (80-120%) and precision (CV < 15%), respectively. Good linearity for most analytes was obtained with R
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.