Evidence map›Paper›PMID 30406159›Full record

ArticleBio-protocol2018

Mammalian Cell-derived Vesicles for the Isolation of Organelle Specific Transmembrane Proteins to Conduct Single Molecule Studies.

Faruk H Moonschi, Ashley M Fox-Loe, Xu Fu, Chris I Richards

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Faruk H MoonschiDepartment of Chemistry, University of Kentucky, Lexington, Kentucky, USA.
Ashley M Fox-LoeDepartment of Chemistry, University of Kentucky, Lexington, Kentucky, USA.
Xu FuDepartment of Chemistry, University of Kentucky, Lexington, Kentucky, USA.
Chris I RichardsDepartment of Chemistry, University of Kentucky, Lexington, Kentucky, USA.

Funding

Training in Drug Abuse Related Research.T32DA016176 · NIDA · UNIVERSITY OF KENTUCKY · PI DWOSKIN, LINDA P · 2004 to 2020
$4.0M
Single molecule determination of nAChR structural assembly for therapeutic targetingR33DA038817 · NIDA · UNIVERSITY OF KENTUCKY · PI RICHARDS, CHRISTOPHER I · 2017 to 2018
$759k
Single molecule determination of nAChR structural assembly for therapeutic targetingR21DA038817 · NIDA · UNIVERSITY OF KENTUCKY · PI RICHARDS, CHRISTOPHER I · 2015 to 2016
$372k
NIDA NIH HHS R21 DA038817NIDA NIH HHS R33 DA038817NIDA NIH HHS T32 DA016176
6 · The paper itself

Abstract

Cell-derived vesicles facilitate the isolation of transmembrane proteins in their physiological membrane maintaining their structural and functional integrity. These vesicles can be generated from different cellular organelles producing, housing, or transporting the proteins. Combined with single-molecule imaging, isolated organelle specific vesicles can be employed to study the trafficking and assembly of the embedded proteins. Here we present a method for organelle specific single molecule imaging via isolation of ER and plasma membrane vesicles from HEK293T cells by employing OptiPrep gradients and nitrogen cavitation. The isolation was validated through Western blotting, and the isolated vesicles were used to perform single molecule studies of oligomeric receptor assembly.

Indexed as

ER and plasma membrane protein separationNicotinic receptorOptiPrepPhotobleachingProtein traffickingSingle moleculeStoichiometryVesicles

Identifiers

PMID30406159
PMCPMC6217831

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.