ArticleFrontiers in cellular and infection microbiology2018
Metagenomic Sequencing From Mosquitoes in China Reveals a Variety of Insect and Human Viruses.
Article in Frontiers in cellular and infection microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 34 citations in OpenAlex.
- Development of JEV NS1 Specific Capture-ELISA Based on a Single Monoclonal Antibody.Animals : an open access journal from MDPI · 2026Article
- Identification of insect-specific viruses in mosquitoes collected from wildlife and rural areas in north-eastern parts of South Africa using a metagenomic RNA sequencing approach.One health outlook · 2025Article
- Virome profiling of Aedes albopictus across urban ecosystems in Guangdong reveals sex-specific diversity.Parasites & vectors · 2025Article
- Unveiling viral diversity and dynamics in mosquitoes through metagenomic analysis in Guizhou Province, China.Infectious diseases of poverty · 2025Article
- Characterization of the Virome in Mosquitoes Across Distinct Habitats in the Yucatán Peninsula, Mexico.Viruses · 2025Article
- Metagenomic analysis of mosquitoes from Kangerlussuaq, Greenland reveals a unique virome.Scientific reports · 2025Article
- Sheep serve as amplifying hosts of Japanese encephalitis virus, increasing the risk of human infection.Science advances · 2025Article
- Insect-specific RNA viruses detection in Field-Caught Aedes aegypti mosquitoes from Argentina using NGS technology.PLoS neglected tropical diseases · 2025Article
- An Exploration of the Viral Coverage of Mosquito Viromes Using Meta-Viromic Sequencing: A Systematic Review and Meta-Analysis.Microorganisms · 2024Review
- Tips and tools to obtain and assess mosquito viromes.Archives of microbiology · 2024Review
- Metavirome Analysis and Identification of Midge-Borne Viruses from Yunnan Province, China, in 2021.Viruses · 2023Article
- Composition and global distribution of the mosquito virome - A comprehensive database of insect-specific viruses.One health (Amsterdam, Netherlands) · 2023Article
- Novel PutativeViruses · 2023Article
- Metagenomic analysis of viral diversity and a novel astroviruse of forest rodent.Virology journal · 2022Article
- Aedes aegypti and Ae. albopictus microbiome/virome: new strategies for controlling arboviral transmission?Parasites & vectors · 2022Review
- Toll-like receptor 2 signaling pathway activation contributes to a highly efficient inflammatory response in Japanese encephalitis virus-infected mouse microglial cells by proteomics.Frontiers in microbiology · 2022Article
- Article
- Metavirome Analysis ofFrontiers in cellular and infection microbiology · 2022Article
- Single mosquito metatranscriptomics identifies vectors, emerging pathogens and reservoirs in one assay.eLife · 2021Article
- Considerations for mosquito microbiome research from the Mosquito Microbiome Consortium.Microbiome · 2021Review
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Authors and funding
14 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We collected 8,700 mosquitoes in three sites in China, which belonged to seven species. Their viromes were tested using metagenomic sequencing and bioinformatic analysis. The abundant viral sequences were detected and annotated belonging to more than 50 viral taxonomic families. The results were verified by PCR, followed by phylogenetic analysis. In the present study, we identified partial viral genes of dengue virus (DENV), a novel circovirus (CCV), densovirus (DNV), Japanese encephalitis virus (JEV), and Wuhan mosquito virus (WMV) in mosquitoes. Metagenomic analysis and PCR amplification revealed three DENV sequences, which were as homologous to the NS3 gene of DENV from Singapore isolated in 2005, with at least 91% nucleotide (nt) identity. Seven fragments of JEV encoding structural proteins were identified belonging to genotype I. They all shared high homology with structural protein genes of JEV isolated from Laos in 2009. The production of infectious virus particles of the newly isolated virus YunnanJEV2017-4 increased after passage from the BHK-21 cell line to the Vero cell line. Novel circovirus-related genes were identified and as being related to an unnamed gene of a mosquito circovirus (MCCV) sequence from the USA isolated in 2011, with at least 41% nt identity: this distant relationship suggests that the parent virus might belong to a novel circovirus genus. Additionally, numerous known viruses and some unknown viruses were also detected in mosquitoes from Yunnan province, China, which will be tested for propagation.
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