Evidence map›Paper›PMID 30406038›Full record

ArticleFrontiers in cellular and infection microbiology2018

Metagenomic Analysis of

Pengpeng Xiao, Jicheng Han, Ying Zhang, Chenghui Li, Xiaofang Guo, Shubo Wen, Mingyao Tian, Yiquan Li, Maopeng Wang, Hao Liu and 4 more

Open access · goldAbstract read
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
4.4field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 47 citations in OpenAlex.

  1. Article
  2. Integrating metagenomics and metatranscriptomics intoThe Journal of general virology · 2026
    Review
  3. Article
  4. Mosquito diversity and orthoflavivirus detection in Khao Yai National Park, Thailand.Current research in parasitology & vector-borne diseases · 2026
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Review
  10. Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Review
  17. Article
  18. Review
  19. Metagenomic Analysis ofFrontiers in cellular and infection microbiology · 2022
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 7 institutions in 1 country.

Pengpeng XiaoYanbian University Medical College, Yanji, China.
Jicheng HanYanbian University Medical College, Yanji, China.
Ying ZhangCollege of Veterinary Medicine, College of Animal Science, Jilin University, Changchun, China.
Chenghui LiYanbian University Medical College, Yanji, China.
Xiaofang GuoYunnan Institute of Parasitic Diseases, Simao, China.
Shubo WenInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China.
Mingyao TianInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China.
Yiquan LiYanbian University Medical College, Yanji, China.
Maopeng WangInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China.
Hao LiuInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China.
Jingqiang RenInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China.
Hongning ZhouYunnan Institute of Parasitic Diseases, Simao, China.
Huijun LuInstitute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China.
Ningyi JinYanbian University Medical College, Yanji, China.
Academy of Military Medical Sciences · CNYanbian University · CNYunnan Institute of Parasitic Diseases · CNFoshan University · CNInstitute of Special Animal and Plant Sciences · CNJilin University · CNWenzhou University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

More than 6,000 mosquitoes of six species from six sites were collected and tested for their virome using metagenomics sequencing and bioinformatic analysis. The identified viral sequences belonged to more than 50 viral families. The results were verified by PCR of selected viruses in all mosquitoes, followed by phylogenetic analysis. In the present study, we identified the partial dengue virus (DENV), Zika virus (ZIKV), and Japanese encephalitis virus (JEV) sequences in mosquitoes. Metagenomic analysis and the PCR amplification revealed three DENV sequences, one of which encodes a partial envelope protein. Two ZIKV sequences both encoding partial nonstructural protein 3 and one JEV sequence encoding the complete envelope protein were identified. There was variability in the viral titers of the newly isolated virus JEV-China/YN2016-1 of different passage viruses. The newly identified Zika virus gene from ZIKV-China/YN2016-1 was an Asian genotype and shared the highest nucleotide sequence identity (97.1%) with a ZIKV sequence from Thailand isolated in 2004. Phylogenetic analysis of ZIKV-China/YN2016-1 and ZIKV-China/YN2016-2 with known

Indexed as

Genes, ViralAnimalsChinaComputational BiologyDengue VirusEncephalitis Virus, JapaneseMetagenomicsMosquito VectorsPolymerase Chain ReactionSequence Analysis, DNAZika Virusmetagenomic analysismosquitophylogenetic analysisviromevirus detection

Identifiers

PMID30406038
PMCPMC6207848
OpenAlexW2898355074

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.