ArticleFrontiers in molecular neuroscience2018
Mapping Transgene Insertion Sites Reveals Complex Interactions Between Mouse Transgenes and Neighboring Endogenous Genes.
Article in Frontiers in molecular neuroscience, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 62 citations in OpenAlex.
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- Stable Inheritance of Transgene and Yellow Fluorescent Protein Gene Expression in Progeny of Transgenic Cacao (Plants (Basel, Switzerland) · 2026Article
- Transgene Mapping in Animals: What to Choose?International journal of molecular sciences · 2025Review
- Altered proportions of retinal cell types and distinct visual codes in rodents occupying divergent ecological niches.Current biology : CB · 2025Article
- Synaptic cell adhesion moleculeeLife · 2025Article
- The widely used Ucp1-Cre transgene elicits complex developmental and metabolic phenotypes.Nature communications · 2025Article
- PLP1-Targeting Antisense Oligonucleotides Improve FOXG1 Syndrome Mice.International journal of molecular sciences · 2024Article
- A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types.bioRxiv : the preprint server for biology · 2024Article
- Severe neurodegeneration in brains of transgenic rats producing human tau prions.Acta neuropathologica · 2024Article
- Reconfiguration of the visual code and retinal cell type complement in closely related diurnal and nocturnal mice.bioRxiv : the preprint server for biology · 2024Article
- Identifying transgene insertions inPeerJ · 2024Article
- The widely usedbioRxiv : the preprint server for biology · 2023Article
- CRISPR-Cas9 Long-Read Sequencing for Mapping Transgenes in the Mouse Genome.The CRISPR journal · 2023Article
- A melanopsin ganglion cell subtype forms a dorsal retinal mosaic projecting to the supraoptic nucleus.Nature communications · 2023Article
- Pyramidal cell types drive functionally distinct cortical activity patterns during decision-making.Nature neuroscience · 2023Article
- Transcription-coupled donor DNA expression increases homologous recombination for efficient genome editing.Nucleic acids research · 2022Article
- Unified classification of mouse retinal ganglion cells using function, morphology, and gene expression.Cell reports · 2022Article
- Evaluation of the α-casein (CSN1S1) locus as a potential target for a site-specific transgene integration.Scientific reports · 2022Article
- Mapping transgene insertion sites reveals the α-Cre transgene expression in both developing retina and olfactory neurons.Communications biology · 2022Article
- Annotated expression and activity data for murine recombinase alleles and transgenes: the CrePortal resource.Mammalian genome : official journal of the International Mammalian Genome Society · 2022Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Transgenic mouse lines are routinely employed to label and manipulate distinct cell types. The transgene generally comprises cell-type specific regulatory elements linked to a cDNA encoding a reporter or other protein. However, off-target expression seemingly unrelated to the regulatory elements in the transgene is often observed, it is sometimes suspected to reflect influences related to the site of transgene integration in the genome. To test this hypothesis, we used a proximity ligation-based method, Targeted Locus Amplification (TLA), to map the insertion sites of three well-characterized transgenes that appeared to exhibit insertion site-dependent expression in retina. The nearest endogenous genes to transgenes HB9-GFP, Mito-P, and TYW3 are
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.