ArticleNature communications2018
Kauniolide synthase is a P450 with unusual hydroxylation and cyclization-elimination activity.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
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- Metabolic engineering inaBIOTECH · 2025Review
- Cytochrome P450-Induced Backbone Rearrangements in Terpene Biosynthesis of Plants.Molecules (Basel, Switzerland) · 2025Review
- Plant sesquiterpene lactones.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Recent advances in lycopene and germacrene a biosynthesis and their role as antineoplastic drugs.World journal of microbiology & biotechnology · 2024Review
- Plant terpene specialized metabolism: complex networks or simple linear pathways?The Plant journal : for cell and molecular biology · 2023Review
- Lactucin Synthase Inactivation Boosts the Accumulation of Anti-inflammatory 8-Deoxylactucin and Its Derivatives in Chicory (Journal of agricultural and food chemistry · 2023Article
- Tuning Selectivity in CalA Lipase: Beyond Tunnel Engineering.Biochemistry · 2023Article
- Identification and characterization of CYP71 subclade cytochrome P450 enzymes involved in the biosynthesis of bitterness compounds inFrontiers in plant science · 2023Article
- Cloning and Functional Characterization of Two Germacrene A Oxidases Isolated fromMolecules (Basel, Switzerland) · 2022Article
- Biotransformation of (-)-α-Bisabolol byMolecules (Basel, Switzerland) · 2022Article
- CRISPR/Cas9 targeted inactivation of the kauniolide synthase in chicory results in accumulation of costunolide and its conjugates in taproots.Frontiers in plant science · 2022Article
- Article
- Diverse Functions of IAA-Leucine Resistant PpILR1 Provide a Genic Basis for Auxin-Ethylene Crosstalk During Peach Fruit Ripening.Frontiers in plant science · 2021Article
- Article
- Traps and Pitfalls-Unspecific Reactions in Metabolic Engineering of Sesquiterpenoid Pathways.Molecules (Basel, Switzerland) · 2020Review
- Syntheses of Complex Terpenes from Simple Polyprenyl Precursors.Accounts of chemical research · 2020Review
- Silencing of germacrene A synthase genes reduces guaianolide oxalate content inGM crops & food · 2020Article
- Allylative Approaches to the Synthesis of Complex Guaianolide Sesquiterpenes from Apiaceae and Asteraceae.Journal of the American Chemical Society · 2019Article
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Authors and funding
12 authors at 6 institutions in 3 countries.
Funding
Abstract
Guaianolides are an important class of sesquiterpene lactones with unique biological and pharmaceutical properties. They have been postulated to be derived from germacranolides, but for years no progress has been made in the elucidation of their biosynthesis that requires an unknown cyclization mechanism. Here we demonstrate the isolation and characterization of a cytochrome P450 from feverfew (Tanacetum parthenium), kauniolide synthase. Kauniolide synthase catalyses the formation of the guaianolide kauniolide from the germacranolide substrate costunolide. Unlike most cytochrome P450s, kauniolide synthase combines stereoselective hydroxylation of costunolide at the C3 position, with water elimination, cyclization and regioselective deprotonation. This unique mechanism of action is supported by in silico modelling and docking experiments. The full kauniolide biosynthesis pathway is reconstructed in the heterologous hosts Nicotiana benthamiana and yeast, paving the way for biotechnological production of guaianolide-type sesquiterpene lactones.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.