Evidence map›Paper›PMID 30404012›Full record

ArticleCell reports2018

Brief Local Application of Progesterone via a Wearable Bioreactor Induces Long-Term Regenerative Response in Adult Xenopus Hindlimb.

Celia Herrera-Rincon, Annie S Golding, Kristine M Moran, Christina Harrison, Christopher J Martyniuk, Justin A Guay, Julia Zaltsman, Hayley Carabello, David L Kaplan, Michael Levin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 39 citations in OpenAlex.

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  9. Sex and Regeneration.Biology · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Celia Herrera-RinconBiology Department and Allen Discovery Center, Tufts University, Medford, MA, USA.
Annie S GoldingDepartment of Chemical and Biological Engineering, Tufts University, Medford, MA, USA.
Kristine M MoranBiology Department and Allen Discovery Center, Tufts University, Medford, MA, USA.
Christina HarrisonBiology Department and Allen Discovery Center, Tufts University, Medford, MA, USA.
Christopher J MartyniukCenter for Environmental and Human Toxicology and Department of Physiological Sciences, University of Florida, Gainesville, FL, USA.
Justin A GuayBiology Department and Allen Discovery Center, Tufts University, Medford, MA, USA.
Julia ZaltsmanBiology Department and Allen Discovery Center, Tufts University, Medford, MA, USA.
Hayley CarabelloBiology Department and Allen Discovery Center, Tufts University, Medford, MA, USA.
David L KaplanDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.
Michael LevinBiology Department and Allen Discovery Center, Tufts University, Medford, MA, USA; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA. Electronic address: michael.levin@tufts.edu.
Tufts University · USHarvard University · USUniversity of Florida · US

Funding

Electrotherapeutic strategies for connective tissue repairR01AR061988 · NIAMS · TUFTS UNIVERSITY MEDFORD · PI HUNG, CLARK T., KAPLAN, DAVID L. · 2011 to 2015
$3.3M
Tissue Regeneration by Biophysical SignalingR01AR055993 · NIAMS · TUFTS UNIVERSITY MEDFORD · PI KAPLAN, DAVID L., LEVIN, MICHAEL · 2010 to 2018
$3.2M
NIAMS NIH HHS R01 AR055993NIAMS NIH HHS R01 AR061988
6 · The paper itself

Abstract

The induction of limb repair in adult vertebrates is a pressing, unsolved problem. Here, we characterize the effects of an integrated device that delivers drugs to severed hindlimbs of adult Xenopus laevis, which normally regenerate cartilaginous spikes after amputation. A wearable bioreactor containing a silk protein-based hydrogel that delivered progesterone to the wound site immediately after hindlimb amputation for only 24 hr induced the regeneration of paddle-like structures in adult frogs. Molecular markers, morphometric analysis, X-ray imaging, immunofluorescence, and behavioral assays were used to characterize the differences between the paddle-like structures of successful regenerates and hypomorphic spikes that grew in untreated animals. Our experiments establish a model for testing therapeutic cocktails in vertebrate hindlimb regeneration, identify pro-regenerative activities of progesterone-containing bioreactors, and provide proof of principle of brief use of integrated device-based delivery of small-molecule drugs as a viable strategy to induce and maintain a long-term regenerative response.

Indexed as

BioreactorsWearable Electronic DevicesAction PotentialsAnimalsBone RemodelingGene Regulatory NetworksHindlimbMotor ActivityProgesteroneReceptors, ProgesteroneRegenerationSwimmingTranscriptomeWound HealingXenopus laevisProgesteroneReceptors, Progesteronebioelectricitybioreactorfroghydrogel devicelimbregenerationsilkspikeXenopus

Identifiers

PMID30404012
PMCPMC6317729
OpenAlexW2899590661

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.