ArticleJournal of experimental botany2019
Temperature response of Rubisco kinetics in Arabidopsis thaliana: thermal breakpoints and implications for reaction mechanisms.
Article in Journal of experimental botany, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- Photosynthetic Responses to Rising COGlobal change biology · 2026Review
- Tropical forest trees with higher thermal optima of photosynthesis exhibit lower PSII heat stability.Journal of experimental botany · 2026Article
- Rubisco kinetic acclimation at the holoenzyme level.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Rho GTPase overexpression impacts leaf internal architecture and mesophyll conductance in Arabidopsis.The New phytologist · 2025Article
- Temperature-induced changes in Arabidopsis Rubisco activity and isoform expression.Journal of experimental botany · 2023Article
- Functional reconstitution of a bacterial COeLife · 2020Article
- Big progress for small subunits: new Rubisco mutants in Arabidopsis.Journal of experimental botany · 2020Article
- Measuring Rubisco activity: challenges and opportunities of NADH-linked microtiter plate-based and 14C-based assays.Journal of experimental botany · 2020Article
- Chemical Reaction Networks Possess Intrinsic, Temperature-Dependent Functionality.Entropy (Basel, Switzerland) · 2020Article
- Revisiting Trade-offs between Rubisco Kinetic Parameters.Biochemistry · 2019Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enhancement of Rubisco kinetics could improve photosynthetic efficiency, ultimately resulting in increased crop yield. However, imprecise knowledge of the reaction mechanism and the individual rate constants limits our ability to optimize the enzyme. Membrane inlet mass spectrometry (MIMS) may offer benefits over traditional methods for determining individual rate constants of the Rubisco reaction mechanism, as it can directly monitor concentration changes in CO2, O2, and their isotopologs during assays. However, a direct comparison of MIMS with the traditional radiolabel method of determining Rubisco kinetic parameters has not been made. Here, the temperature responses of Rubisco kinetic parameters from Arabidopsis thaliana were measured using radiolabel and MIMS methods. The two methods provided comparable parameters above 25 °C, but temperature responses deviated at low temperature as MIMS-derived catalytic rates of carboxylation, oxygenation, and CO2/O2 specificity showed thermal breakpoints. Here, we discuss the variability and uncertainty surrounding breakpoints in the Rubisco temperature response and the relevance of individual rate constants of the reaction mechanisms to potential breakpoints.
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