Evidence map›Paper›PMID 30402716›Full record

ArticleVeterinary research communications2019

Optimised isolation method for RNA extraction suitable for RNA sequencing from feline teeth collected in a clinical setting and at post mortem.

S Lee, U Trivedi, C Johnson, C Farquharson, G T Bergkvist

Erratum issuedOpen access · hybridAbstract read
PubMed Publisher
In one paragraph

Article in Veterinary research communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Role of Notch, IL-1 and leptin expression in colorectal cancer.Experimental and therapeutic medicine · 2021
    Article
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

S LeeThe Royal (Dick) School of Veterinary Studies and the Roslin Institute, The University of Edinburgh, Easter Bush Campus, Midlothian, EH25 9RG, UK. seungmee.lee@roslin.ed.ac.uk.ORCID http://orcid.org/0000-0002-3460-9987
U TrivediEdinburgh Genomics, The University of Edinburgh, Charlotte Auerbach Road, Edinburgh, EH9 3FL, UK.
C JohnsonCentre for Applied Anatomy, University of Bristol, Southwell Street, Bristol, BS2 8EJ, UK.
C FarquharsonThe Royal (Dick) School of Veterinary Studies and the Roslin Institute, The University of Edinburgh, Easter Bush Campus, Midlothian, EH25 9RG, UK.
G T BergkvistThe Royal (Dick) School of Veterinary Studies and the Roslin Institute, The University of Edinburgh, Easter Bush Campus, Midlothian, EH25 9RG, UK.
Roslin Institute · GBEdinburgh Genomics · GBUniversity of Bristol · GB

Funding

Biotechnology and Biological Sciences Research Council R42604Edinburgh Global Research Scholarship, The University of Edinburgh R43199Fiona and Ian Russell Seed Corn Grant Fund G31335
6 · The paper itself

Abstract

Advanced next generation sequencing approaches have started to reveal the cellular and molecular complexity of the microenvironment in many tissues. It is challenging to obtain high quality RNA from mineralised tissues. We developed an optimised method of RNA extraction from feline teeth collected in a clinical setting and at post mortem. Teeth were homogenised in phenol-guanidinium solution at near-freezing temperatures and followed by solid-phase nucleic acid extraction utilising a commercially available kit. This method produced good RNA yields and improved RNA quality based on RNA integrity numbers equivalent (RIN

Indexed as

AnimalsCadaverCat DiseasesCatsPolymerase Chain ReactionRNASequence Analysis, RNAToothTooth ResorptionRNAFeline toothGene expressionRNA extractionRNA integrityRNA purityRNA sequencing

Identifiers

PMID30402716
OpenAlexW2900100227

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.