ArticleChemistryOpen2018
Direct Observation of Bacterial Growth in Giant Unilamellar Vesicles: A Novel Tool for Bacterial Cultures.
Article in ChemistryOpen, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed.
- Microfluidic Giant Polymer Vesicles Equipped with Biopores for High-Throughput Screening of Bacteria.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Cell-sized asymmetric phospholipid-amphiphilic protein vesicles with growth, fission, and molecule transportation.iScience · 2023Article
- Cell-Derived Vesicles with Increased Stability and On-Demand Functionality by Equipping Their Membrane with a Cross-Linkable Copolymer.Advanced healthcare materials · 2022Article
- Encapsulated bacteria deform lipid vesicles into flagellated swimmers.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Rapid and Facile Preparation of Giant Vesicles by the Droplet Transfer Method for Artificial Cell Construction.Frontiers in bioengineering and biotechnology · 2022Article
- Lipid in Chips: A Brief Review of Liposomes Formation by Microfluidics.International journal of nanomedicine · 2021Review
- Bacterial Behavior in Confined Spaces.Frontiers in cell and developmental biology · 2021Review
- Session 1SCA-Utilizing soft compartments/interfaces for the creation of artificial biosystems.Biophysical reviews · 2020Article
- Creation of Artificial Cell-Like Structures Promoted by Microfluidics Technologies.Micromachines · 2019Review
- Direct Observation of Bacterial Growth in Giant Unilamellar Vesicles: A Novel Tool for Bacterial Cultures.ChemistryOpen · 2018Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial cultivation techniques are classic, basic, and common processes used to characterize the physiological activity of bacteria in their environment. Owing to recent advances in bacterial cultivation techniques, the physiological activity of bacteria can be elucidated at the single-cell culture level. Here, we report a novel method to monitor the real-time activity of bacterial growth at the single-cell level inside giant unilamellar vesicles (GUVs). This method consists of two steps: 1) encapsulation of single bacteria in 1-33 pL scale GUVs and 2) immobilization of the GUVs on a planar lipid bilayer membrane on a glass surface. We directly observed single
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.