ArticleBiomedical reports2018
A method for extracting DNA from hard tissues for use in forensic identification.
Article in Biomedical reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Impact of Semi-Permanent Nail Polish on Forensic DNA Profiling and Phenotyping from Fingernails.Genes · 2026Article
- Assessing the Micro- and Macroscopic Changes of Chemically Altered Human Bone and Teeth.Biomolecules · 2025Article
- Regional estimation by oxygen isotope ratio analysis using tooth enamel of Japanese individuals.Forensic sciences research · 2025Article
- Assessment of the genetic diversity of Atlantic bottlenose dolphin (Tursiops truncatus) strandings in the Mississippi Sound (USA).PloS one · 2025Article
- Evaluation of β-Actin and Mitochondrial DNA Levels in Determining the Age ofInternational journal of molecular sciences · 2024Article
- Investigation of a DNA Profiling Method Using Only Cementum More Than 70 Years After Death.Cureus · 2024Article
- Diagnostic and Therapeutic Potential of Circulating-Free DNA and Cell-Free RNA in Cancer Management.Biomedicines · 2022Review
- The human intervertebral disc as a source of DNA for molecular identification.Forensic science, medicine, and pathology · 2021Article
- Epidemiological analysis of African swine fever in the European Union (September 2019 to August 2020).EFSA journal. European Food Safety Authority · 2021Article
- Comparative forensic analysis of reverse root canal filing and conventional method for DNA isolation from extracted teeth under different environmental conditions: A prospective study.The Journal of forensic odonto-stomatology · 2020Article
- Evaluation of Parameters for Estimating the Postmortem Interval of Skeletal Remains Using Bovine Femurs: A Pilot Study.Diagnostics (Basel, Switzerland) · 2020Article
- Forensic validation of a panel of 12 SNPs for identification of Mongolian wolf and dog.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With deceased and decayed bodies, personal identification is performed using hard tissue DNA, commonly extracted from bone. The quantity and quality of DNA used in the polymerase chain reaction (PCR) amplification step is critical for a successful outcome. Since enamel is the strongest tissue in the human body, it was hypothesized that teeth may preserve DNA better than bones. In the present study, porcine teeth and bone samples were exposed to a variety of environments that imitated personal identification conditions, and DNA extracted from the teeth and bone samples was compared, using a PCR amplification method. The porcine teeth and bones were exposed to 11 different conditions for 5 different time periods to imitate a series of common crime scenes. DNA was extracted by a standard phenol-chloroform method. To test DNA quality, PCR was performed with primers designed to amplify porcine β-actin (
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