ArticleLaboratory investigation; a journal of technical methods and pathology2019
Nondestructive, multiplex three-dimensional mapping of immune infiltrates in core needle biopsy.
Article in Laboratory investigation; a journal of technical methods and pathology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Article
- Increased Multiplexity in Optical Tissue Clearing-Based Three-Dimensional Immunofluorescence Microscopy of the Tumor Microenvironment by Light-Emitting Diode Photobleaching.Laboratory investigation; a journal of technical methods and pathology · 2024Article
- Direct three-dimensional segmentation of prostate glands with nnU-Net.Journal of biomedical optics · 2024Article
- Multiresolution 3D Optical Mapping of Immune Cell Infiltrates in Mouse Asthmatic Lung.American journal of respiratory cell and molecular biology · 2023Article
- Multidimensional Imaging of Breast Cancer.Cold Spring Harbor perspectives in medicine · 2023Review
- Article
- 3D imaging for driving cancer discovery.The EMBO journal · 2022Review
- Prostate Cancer Risk Stratification via Nondestructive 3D Pathology with Deep Learning-Assisted Gland Analysis.Cancer research · 2022Article
- Large-scale, three-dimensional tissue cytometry of the human kidney: a complete and accessible pipeline.Laboratory investigation; a journal of technical methods and pathology · 2021Article
- Harnessing non-destructive 3D pathology.Nature biomedical engineering · 2021Review
- Multiplexed Tissue Tomography.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Combined transmission, dark field and fluorescence microscopy for intact, 3D tissue analysis of biopsies.Journal of biomedical optics · 2020Article
- Deep learning-based image analysis methods for brightfield-acquired multiplex immunohistochemistry images.Diagnostic pathology · 2020Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Enumeration of tumor-infiltrating lymphocytes (TILs) in H&E stained tissue sections has demonstrated limited value in predicting immune responses to cancer immunotherapy, likely reflecting the diversity of cell types and immune activation states among tumor infiltrates. Multiparametric flow cytometry enables robust phenotypic and functional analysis to distinguish suppression from activation, but tissue dissociation eliminates spatial context. Multiplex methods for immunohistochemistry (IHC) are emerging, but these interrogate only a single tissue section at a time. Here, we report transparent tissue tomography (T3) as a tool for three-dimensional (3D) imaging cytometry in the complex architecture of the tumor microenvironment, demonstrating multiplexed immunofluorescent analysis in core needle biopsies. Using T3 imaging, image processing and machine learning to map CD3
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.