Evidence map›Paper›PMID 30401626›Full record

ArticleInsect biochemistry and molecular biology2018

Reverse chemical ecology-based approach leading to the accidental discovery of repellents for Rhodnius prolixus, a vector of Chagas diseases refractory to DEET.

Thiago A Franco, Pingxi Xu, Nathália F Brito, Daniele S Oliveira, Xiaolan Wen, Monica F Moreira, C Rikard Unelius, Walter S Leal, Ana C A Melo

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Insect biochemistry and molecular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
2.9field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 36 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 3 countries.

Thiago A FrancoUniversidade Federal do Rio de Janeiro, Instituto de Química, 21941-909, Rio de Janeiro, RJ, Brazil; Department of Molecular and Cellular Biology, University of California, Davis, CA, 95616, USA.
Pingxi XuDepartment of Molecular and Cellular Biology, University of California, Davis, CA, 95616, USA.
Nathália F BritoUniversidade Federal do Rio de Janeiro, Instituto de Química, 21941-909, Rio de Janeiro, RJ, Brazil.
Daniele S OliveiraUniversidade Federal do Rio de Janeiro, Instituto de Química, 21941-909, Rio de Janeiro, RJ, Brazil.
Xiaolan WenDepartment of Molecular and Cellular Biology, University of California, Davis, CA, 95616, USA.
Monica F MoreiraUniversidade Federal do Rio de Janeiro, Instituto de Química, 21941-909, Rio de Janeiro, RJ, Brazil; Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular, Rio de Janeiro, RJ, Brazil.
C Rikard UneliusFaculty of Health and Life Sciences, Linnaeus University, Kalmar, Sweden.
Walter S LealDepartment of Molecular and Cellular Biology, University of California, Davis, CA, 95616, USA. Electronic address: wsleal@ucdavis.edu.
Ana C A MeloUniversidade Federal do Rio de Janeiro, Instituto de Química, 21941-909, Rio de Janeiro, RJ, Brazil; Department of Molecular and Cellular Biology, University of California, Davis, CA, 95616, USA; Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular, Rio de Janeiro, RJ, Brazil. Electronic address: anamelo@iq.ufrj.br.
Universidade Federal do Rio de Janeiro · BRUniversity of California, Davis · USLinnaeus University · SE

Funding

Olfactory protein-based screening of oviposition attractants for Culex mosquitoesR01AI095514 · NIAID · UNIVERSITY OF CALIFORNIA AT DAVIS · PI LEAL, WALTER S · 2012 to 2021
$3.6M
NIAID NIH HHS R01 AI095514
6 · The paper itself

Abstract

Rhodnius prolixus is one of the most important vectors of Chagas disease in Central and South America for which repellents and attractants are sorely needed. Repellents like DEET, picaridin, and IR3535 are widely used as the first line of defense against mosquitoes and other vectors, but they are ineffective against R. prolixus. Our initial goal was to identify in R. prolixus genome odorant receptors sensitive to putative sex pheromones. We compared gene expression of 21 ORs in the R. prolixus genome, identified 4 ORs enriched in male (compared with female) antennae. Attempts to de-orphanize these ORs using the Xenopus oocyte recording system showed that none of them responded to putative sex pheromone constituents. One of the them, RproOR80, was sensitive to 4 compounds in our panel of 109 odorants, namely, 2-heptanone, γ-octalactone, acetophenone, and 4-methylcychohexanol. Interestingly, these compounds, particularly 4-methylcyclohexanol, showed strong repellency activity as indicated not only by a significant decrease in residence time close to a host, but also by a remarkable reduction in blood intake. 4-Methylcyclohexanol-elicited repellency activity was abolished in RNAi-treated insects. In summary, our search for pheromone receptors led to the discovery of repellents for R. prolixus.

Indexed as

AcetophenonesAnimalsArthropod AntennaeChagas DiseaseCyclohexanolsFemaleInsect RepellentsInsect VectorsKetonesLactonesMaleReceptors, OdorantRhodnius2-heptanone4-methylcyclohexanolacetophenoneAcetophenonesCyclohexanolsInsect RepellentsKetonesLactonesReceptors, Odorant2-Heptanone4-MethylcyclohexanolAcetophenoneDEETOdorant receptorRepellentRhodnius prolixusRNAiγ-Octalactone

Identifiers

PMID30401626
PMCPMC6278923
OpenAlexW2898145842

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.