ArticleBMC genomics2018
Correcting palindromes in long reads after whole-genome amplification.
Article in BMC genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Fragmentation of Long Reads Enables Reliable Mitogenome Assembly From Whole-Genome Amplification Data With Pervasive Palindromic Reads.Molecular ecology resources · 2026Article
- Targeted and complete genomic sequencing of the major histocompatibility complex in haplotypic form of individual heterozygous samples.Genome research · 2024Article
- Unveiling microbial diversity: harnessing long-read sequencing technology.Nature methods · 2024Review
- Characterisation and comparative genomics of three new Varanus-associated Borrelia spp. from Indonesia and Australia.Parasites & vectors · 2023Article
- Single-worm long-read sequencing reveals genome diversity in free-living nematodes.Nucleic acids research · 2023Article
- A mini-TGA protein modulates gene expression through heterogeneous association with transcription factors.Plant physiology · 2023Article
- Population-wide gene disruption in the murine lung epithelium via AAV-mediated delivery of CRISPR-Cas9 components.Molecular therapy. Methods & clinical development · 2022Article
- Accurate long-read sequencing allows assembly of the duplicated RHD and RHCE genes harboring variants relevant to blood transfusion.American journal of human genetics · 2022Article
- Article
- Long-read metagenomics of multiple displacement amplified DNA of low-biomass human gut phageomes by SACRA pre-processing chimeric reads.DNA research : an international journal for rapid publication of reports on genes and genomes · 2021Article
- Exact mapping of Illumina blind spots in theMicrobial genomics · 2021Article
- PB-Motif-A Method for Identifying Gene/Pseudogene Rearrangements With Long Reads: An Application to CYP21A2 Genotyping.Frontiers in genetics · 2021Article
- Dynamic evolution of great ape Y chromosomes.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Can we use it? On the utility of de novo and reference-based assembly of Nanopore data for plant plastome sequencing.PloS one · 2020Article
- Complete Sequence and Annotation of the Mycoplasma phocicerebrale Strain 1049Microbiology resource announcements · 2019Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
backgroundNext-generation sequencing requires sufficient DNA to be available. If limited, whole-genome amplification is applied to generate additional amounts of DNA. Such amplification often results in many chimeric DNA fragments, in particular artificial palindromic sequences, which limit the usefulness of long sequencing reads.
resultsHere, we present Pacasus, a tool for correcting such errors. Two datasets show that it markedly improves read mapping and de novo assembly, yielding results similar to these that would be obtained with non-amplified DNA.
conclusionsWith Pacasus long-read technologies become available for sequencing targets with very small amounts of DNA, such as single cells or even single chromosomes.
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