ArticleGenome biology and evolution2018
Conjugative Transposons and Their Cargo Genes Vary across Natural Populations of Rickettsia buchneri Infecting the Tick Ixodes scapularis.
Article in Genome biology and evolution, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- Genomic evolution and adaptation of arthropod-associated Rickettsia.Scientific reports · 2022Pooled it
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- Genomic and Proteomic Analyses of Bacterial Communities ofMicroorganisms · 2025Article
- Development of a Recombinase-Mediated Cassette Exchange System for Gene Knockout and Expression of Non-Native Gene Sequences inVaccines · 2025Article
- Bacterial reprogramming of tick metabolism impacts vector fitness and susceptibility to infection.Nature microbiology · 2024Article
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- Metagenomics of culture isolates and insect tissue illuminate the evolution ofMicrobial genomics · 2023Article
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- Metagenome diversity illuminates origins of pathogen effectors.bioRxiv : the preprint server for biology · 2023Article
- One to host them all: genomics of the diverse bacterial endosymbionts of the spiderMicrobial genomics · 2023Article
- Coinfection of TwoMicrobiology spectrum · 2022Article
- Comparative Whole Genome Analysis of anPathogens (Basel, Switzerland) · 2022Article
- Genomic diversity across the Rickettsia and 'Candidatus Megaira' genera and proposal of genus status for the Torix group.Nature communications · 2022Article
- Cryptic Genes for Interbacterial Antagonism DistinguishFrontiers in cellular and infection microbiology · 2022Article
- Article
- Unpacking the intricacies of Rickettsia-vector interactions.Trends in parasitology · 2021Review
- Cells within cells: Rickettsiales and the obligate intracellular bacterial lifestyle.Nature reviews. Microbiology · 2021Review
- Lipid A Structural Divergence inmSphere · 2021Article
- Growth Dynamics and Antibiotic Elimination of Symbiotic Rickettsia buchneri in the Tick Ixodes scapularis (Acari: Ixodidae).Applied and environmental microbiology · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Rickettsia buchneri (formerly Rickettsia endosymbiont of Ixodes scapularis, or REIS) is an obligate intracellular endoparasite of the black-legged tick, the primary vector of Lyme disease in North America. It is noteworthy among the rickettsiae for its relatively large genome (1.8 Mb) and extraordinary proliferation of mobile genetic elements (MGEs), which comprise nearly 35% of its genome. Previous analysis of the R. buchneri genome identified several integrative conjugative elements named Rickettsiales amplified genomic elements (RAGEs); the composition of these RAGEs suggests that continued genomic invasions by MGEs facilitated the proliferation of rickettsial genes related to an intracellular lifestyle. In this study, we compare the genomic diversity at RAGE loci among sequenced rickettsiae that infect three related Ixodes spp., including two strains of R. buchneri and Rickettsia endosymbiont of Ixodes pacificus strain Humboldt, as well as a closely related species R. tamurae infecting Amblyomma testudinarium ticks. We further develop a novel multiplex droplet digital PCR assay and use it to quantify copy number ratios of chromosomal R. buchneri RAGE-A and RAGE-B to the single-copy gene gltA within natural populations of I. scapularis. Our results reveal substantial diversity among R. buchneri at these loci, both within individual ticks as well as in the I. scapularis population at large, demonstrating that genomic rearrangement of MGEs is an active process in these intracellular bacteria.
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