ArticleScientific data2018
Comprehensive transcriptome analysis of Sarcophaga peregrina, a forensically important fly species.
Article in Scientific data, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Estimating Post-Feeding Developmental Time ofInsects · 2026Article
- Transcriptomic profiling of different developmental stages reveals parasitic strategies of Wohlfahrtia magnifica, a myiasis-causing flesh fly.BMC genomics · 2024Article
- Development of Forensically ImportantAnimals : an open access journal from MDPI · 2023Article
- Characterizing Hox genes in mayflies (Ephemeroptera), with Hexagenia limbata as a new mayfly model.EvoDevo · 2022Article
- Temporal Expression Profiles Reveal Potential Targets during Postembryonic Development of Forensically ImportantInsects · 2022Article
- An annotated checklist of the eukaryotic parasites of humans, exclusive of fungi and algae.ZooKeys · 2021Article
- Molecular Signatures of Sinus Node Dysfunction Induce Structural Remodeling in the Right Atrial Tissue.Molecules and cells · 2020Article
- Genome and Ontogenetic-Based Transcriptomic Analyses of the Flesh Fly,G3 (Bethesda, Md.) · 2019Article
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Authors and funding
8 authors.
Funding
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Abstract
Sarcophaga peregrina (flesh fly) is a frequently found fly species in Palaearctic, Oriental, and Australasian regions that can be used to estimate minimal postmortem intervals important for forensic investigations. Despite its forensic importance, the genome information of S. peregrina has not been fully described. Therefore, we generated a comprehensive gene expression dataset using RNA sequencing and carried out de novo assembly to characterize the S. peregrina transcriptome. We obtained precise sequence information for RNA transcripts using two different methods. Based on primary sequence information, we identified sets of assembled unigenes and predicted coding sequences. Functional annotation of the aligned unigenes was performed using the UniProt, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes databases. As a result, 26,580,352 and 83,221 raw reads were obtained using the Illumina MiSeq and Pacbio RS II Iso-Seq sequencing applications, respectively. From these reads, 55,730 contigs were successfully annotated. The present study provides the resulting genome information of S. peregrina, which is valuable for forensic applications.
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