ArticleLangmuir : the ACS journal of surfaces and colloids2019
Interfacial Interactions of Sucrose during Cryopreservation Detected by Raman Spectroscopy.
Article in Langmuir : the ACS journal of surfaces and colloids, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 49 citations in OpenAlex.
- Natural killer cell cryopreservation: Influence of composition, temperature, and granules on post-thaw survival and function.Molecular therapy. Advances · 2026Article
- Antibacterial effect of chlorogenic acid against Escherichia coli and Lactiplantibacillus plantarum: experimental and molecular dynamic study.World journal of microbiology & biotechnology · 2026Article
- Raman spectroscopic analysis of intracellular ice-induced degradation of mesenchymal stromal cells.Biophysical journal · 2026Article
- Article
- Non-invasive applications of Raman spectroscopy in assisted reproduction.Frontiers in endocrinology · 2025Review
- Evaluation of Cold Resistance in Alfalfa Varieties Based on Root Traits and Winter Survival in Horqin Sandy Land.Biology · 2024Article
- Sucrose Treatment Enhances the Electrotransfer of DNA by Activating Phospholipase A2.Pharmaceutics · 2024Article
- Fully Integrated Microfluidic Platform for Multiplexed Detection of Hunov by a Dynamic Confined-Space-Implemented One-Pot Rpa-Lamp System.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Usage of ectoine as a cryoprotectant for cryopreservation of lactic acid bacteria.Folia microbiologica · 2024Article
- Observation and Measurement of Ice Morphology in Foods: A Review.Foods (Basel, Switzerland) · 2023Review
- Characterizing cellular membrane partitioning of DMSO using low-temperature Raman spectroscopy.Frontiers in molecular biosciences · 2023Article
- Optimization of storage conditions for lipid nanoparticle-formulated self-replicating RNA vaccines.Journal of controlled release : official journal of the Controlled Release Society · 2023Article
- Development of Serum-Free Media for Cryopreservation of Hydrogel Encapsulated Cell-Based Therapeutics.Cellular and molecular bioengineering · 2022Article
- Non-Uniform Distribution of Cryoprotecting Agents in Rice Culture Cells Measured by CARS Microscopy.Plants (Basel, Switzerland) · 2021Article
- Natural deep eutectic systems for nature-inspired cryopreservation of cells.AIChE journal. American Institute of Chemical Engineers · 2021Article
- Raman Cryomicroscopic Imaging and Sample Holder for Spectroscopic Subzero Temperature Measurements.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Understanding the freezing responses of T cells and other subsets of human peripheral blood mononuclear cells using DSMO-free cryoprotectants.Cytotherapy · 2020Article
- Cryopreservation of Human iPS Cell Aggregates in a DMSO-Free Solution-An Optimization and Comparative Study.Frontiers in bioengineering and biotechnology · 2020Article
- Cryopreservation of Hematopoietic Stem Cells: Emerging Assays, Cryoprotectant Agents, and Technology to Improve Outcomes.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2019Review
- Characterizing modes of action and interaction for multicomponent osmolyte solutions on Jurkat cells.Biotechnology and bioengineering · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
There is considerable interest in the use of sugars to preserve cells. In this study, low temperature Raman spectroscopy was used to characterize the behaviors of sucrose during freezing. The hydrogen bond network between sucrose and water was investigated at -10 °C and -50 °C, and the Raman spectra showed strengthened sucrose-water and sucrose-sucrose hydrogen bonds in more concentrated sucrose solution at -50 °C. The concentration of sucrose at the ice interface increased as the ice density decreased, and it plateaued across a narrow channel of nonfrozen sucrose solution before it decreased toward the next ice interface. The biophysical environment at interfaces between the cell and nonfrozen sucrose solution and between the cell and extracellular ice was also studied. A thin layer of nonfrozen sucrose solution was observed at the interface between the cell and extracellular ice. The extracellular concentration of sucrose at this interface was generally lower than that of bulk nonfrozen sucrose solution. The variation of sucrose concentration outside different regions of the cell membrane suggests that the chemical environment around the cell during freezing may be more heterogeneous than previously thought. Raman spectra and images also showed colocalization of nonfrozen sucrose solution and the cell, which implied that direct interaction between sucrose and cell membrane might be responsible for protective properties of sucrose. Sucrose was predominantly distributed outside the cell, and the observation of strong partitioning of sucrose across the cell membrane is consistent with substantial cell dehydration detected by the Raman spectra. This work enhances our understanding of the behaviors of sucrose solution and its interactions with cells at low temperature and can improve cryopreservation protocols of cells frozen in a sucrose-based media.
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