ArticleJournal of assisted reproduction and genetics2019
Histone modification signatures in human sperm distinguish clinical abnormalities.
Article in Journal of assisted reproduction and genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
33 citing papers in PubMed, 65 citations in OpenAlex.
- Sperm Testing in Assisted Reproductive Technology: Diagnosis and Outcome Prediction.Reproductive sciences (Thousand Oaks, Calif.) · 2026Review
- From localization to function: comparative analysis of CB1 in sperm across species and its epigenetic role in humans.Cell death & disease · 2026Article
- Experimental Evidence That Prenatal and Postnatal Developmental Stress Affects the Adult Seminal Fluid Proteome in a Precocial Bird.Molecular ecology · 2026Article
- Proteomic Analysis of Histone Sequence Variants and Post-translationally Modified Forms.Advances in experimental medicine and biology · 2026Review
- Sperm H3K9me3 levels are associated with embryo developmental dynamics and biochemical pregnancy in IVF patients with normozoospermia.Reproductive biology and endocrinology : RB&E · 2025Article
- Protamine lacunae preserve the paternal chromatin landscape in sperm.bioRxiv : the preprint server for biology · 2025Article
- Beyond Genes: Mechanistic and Epidemiological Insights into Paternal Environmental Influence on Offspring Health.Current environmental health reports · 2025Review
- Unraveling the mysteries of early embryonic arrest: genetic factors and molecular mechanisms.Journal of assisted reproduction and genetics · 2024Review
- 17α-Ethynylestradiol alters testicular epigenetic profiles and histone-to-protamine exchange in mice.Reproductive biology and endocrinology : RB&E · 2024Article
- Sperm histone modifications may predict success in human assisted reproduction: a pilot study.Journal of assisted reproduction and genetics · 2024Article
- Review
- Sperm epigenetics and male infertility: unraveling the molecular puzzle.Human genomics · 2024Review
- Article
- Etiology of Male Infertility: an Update.Reproductive sciences (Thousand Oaks, Calif.) · 2024Review
- Article
- Contribution of the paternal histone epigenome to the preimplantation embryo.Frontiers in cell and developmental biology · 2024Review
- Bull Sperm SWATH-MS-Based Proteomics Reveals Link between High Fertility and Energy Production, Motility Structures, and Sperm-Oocyte Interaction.Journal of proteome research · 2023Article
- Nucleosomes in mammalian sperm: conveying paternal epigenetic inheritance or subject to reprogramming between generations?Current opinion in genetics & development · 2023Review
- Towards a Multi-Omics of Male Infertility.The world journal of men's health · 2023Review
- Molecular Markers: A New Paradigm in the Prediction of Sperm Freezability.International journal of molecular sciences · 2023Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
purposeAlternations to the paternal epigenome, specifically the components of sperm chromatin, can lead to infertility in humans and potentially transmit aberrant information to the embryo. One key component of sperm chromatin is the post-translational modification of histones (PTMs). We previously identified a comprehensive profile of histone PTMs in normozoospermic sperm; however, only specific histone PTMs have been identified in abnormal sperm by antibody-based approaches and comprehensive changes to histone PTM profiles remain unknown. Here, we investigate if sperm with abnormalities of total motility, progressive motility, and morphology have altered histone PTM profiles compared to normozoospermic sperm samples.
methodsDiscarded semen samples from 31 men with normal or abnormal semen parameters were analyzed for relative abundance of PTMs on histone H3 and H4 by "bottom-up" nano-liquid chromatography-tandem mass spectrometry.
resultsAsthenoteratozoospermic samples (abnormal motility, forward progression, and morphology, n = 6) displayed overall decreased H4 acetylation (p = 0.001) as well as alterations in H4K20 (p = 0.003) and H3K9 methylation (p < 0.04) when compared to normozoospermic samples (n = 8). Asthenozoospermic samples (abnormal motility and progression, n = 5) also demonstrated decreased H4 acetylation (p = 0.04) and altered H4K20 (p = 0.005) and H3K9 methylation (p < 0.04). Samples with isolated abnormal progression (n = 6) primarily demonstrated decreased acetylation on H4 (p < 0.02), and teratozoospermic samples (n = 6) appeared similar to normozoospermic samples (n = 8).
conclusionSperm samples with combined and isolated abnormalities of total motility, progressive motility, and morphology display distinct and altered histone PTM signatures compared to normozoospermic sperm. This provides evidence that alterations in histone PTMs may be important for normal sperm function and fertility.
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Registered trials
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