ArticleNature methods2018
Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure.
Article in Nature methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
64 citing papers in PubMed, 120 citations in OpenAlex.
- Engineering Antibodies into Targeted Chimeras: From Recognition Modules to Programmable Degraders.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Detection of Target Molecules Within One-Millimeter-Thick Mouse Brain Slices by Using Peroxidase-Fused Nanobodies and Fluorochromized Tyramide-Glucose Oxidase Reaction.Bio-protocol · 2026Article
- Probing molecular diversity and ultrastructure of brain cells with fluorescent aptamers.Nature communications · 2026Article
- 3D STED Super-Resolution Imaging Strategy for Visualizing Synaptic Nano-architecture in Brain Cryosections.Bio-protocol · 2026Article
- The axon initial segment-associated microglia regulate neuronal activity and visual perception.Cell research · 2026Article
- Tiny tools closing the gap: nanobodies in research and therapy.Function (Oxford, England) · 2026Review
- Lrrn-mediated retinal ganglion cell targeting drives visual circuit assembly for brightness and contrast detection.Science advances · 2026Article
- Emergence of Functional Heart-Brain Circuits in a Vertebrate.bioRxiv : the preprint server for biology · 2025Article
- A three dimensional immunolabeling method with peroxidase-fused nanobodies and fluorochromized tyramide-glucose oxidase signal amplification.Communications biology · 2025Article
- Article
- Penta-ALFA-Tagged Substrates for Self-Labelling Tags Allow Signal Enhancement in Microscopy.Journal of peptide science : an official publication of the European Peptide Society · 2025Article
- Combining nanobody labeling with STED microscopy reveals input-specific and layer-specific organization of neocortical synapses.PLoS biology · 2025Article
- Multiplexed volumetric CLEM enabled by scFvs provides insights into the cytology of cerebellar cortex.Nature communications · 2024Article
- Tracing nerve fibers with volume electron microscopy to quantitatively analyze brain connectivity.Communications biology · 2024Article
- Generation of nanobodies from transgenic 'LamaMice' lacking an endogenous immunoglobulin repertoire.Nature communications · 2024Article
- Extensive soma-soma plate-like contact sites (ephapses) connect suprachiasmatic nucleus neurons.The Journal of comparative neurology · 2024Article
- Morphomics via next-generation electron microscopy.Journal of molecular cell biology · 2024Review
- In-section Click-iT detection and super-resolution CLEM analysis of nucleolar ultrastructure and replication in plants.Nature communications · 2024Article
- MICA-specific nanobodies for diagnosis and immunotherapy of MICAFrontiers in immunology · 2024Article
- A novel optimized pre-embedding antibody-labelling correlative light electron microscopy technique.Access microbiology · 2024Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Morphological and molecular characteristics determine the function of biological tissues. Attempts to combine immunofluorescence and electron microscopy invariably compromise the quality of the ultrastructure of tissue sections. We developed NATIVE, a correlated light and electron microscopy approach that preserves ultrastructure while showing the locations of multiple molecular moieties, even deep within tissues. This technique allowed the large-scale 3D reconstruction of a volume of mouse hippocampal CA3 tissue at nanometer resolution.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.