ArticleThe EMBO journal2018
Ultrafast optogenetic stimulation of the auditory pathway by targeting-optimized Chronos.
Article in The EMBO journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
49 citing papers in PubMed, 107 citations in OpenAlex.
- Optogenetic circuit mapping reveals connectivity and synaptic physiology of T-stellate projections from the cochlear nucleus to the auditory midbrain.bioRxiv : the preprint server for biology · 2026Article
- Efficient and sustained optogenetic control of sensory and cardiac systems.Nature biomedical engineering · 2026Article
- Channelrhodopsin variants for high-rate optogenetic neurostimulation at low light intensities.EMBO molecular medicine · 2026Article
- Optogenetic Infection and Optical Stimulation: A Study on Auditory Responses in Guinea Pig Cochlear Neurons.Journal of otology · 2025Article
- Delivery of AAV vectors to the superior olivary complex enables efficient adult cochlear transduction via axonal transport.Molecular therapy : the journal of the American Society of Gene Therapy · 2025Article
- Study on Recovery Strategy of Hearing Loss & SGN Regeneration Under Physical Regulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Improved optogenetic modification of spiral ganglion neurons for future optical cochlear implants.Theranostics · 2025Article
- Article
- Combined-electrical optogenetic stimulation but not channelrhodopsin kinetics improves the fidelity of high rate stimulation in the auditory pathway in mice.Scientific reports · 2024Article
- Acoustic stimulation of the human round window by laser-induced nonlinear optoacoustics.Scientific reports · 2024Article
- Decreasing the physical gap in the neural-electrode interface and related concepts to improve cochlear implant performance.Frontiers in neuroscience · 2024Review
- Optogenetics: Illuminating the Future of Hearing Restoration and Understanding Auditory Perception.Current gene therapy · 2024Review
- Optical measurement of glutamate release robustly reports short-term plasticity at a fast central synapse.Frontiers in molecular neuroscience · 2024Article
- Review
- Channelrhodopsin fluorescent tag replacement for clinical translation of optogenetic hearing restoration.Molecular therapy. Methods & clinical development · 2023Article
- Advanced Temporally-Spatially Precise Technologies for On-Demand Neurological Disorder Intervention.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Towards translational optogenetics.Nature biomedical engineering · 2023Review
- Differential optogenetic activation of the auditory midbrain in freely moving behaving mice.Frontiers in systems neuroscience · 2023Article
- Signal processing and stimulation potential within the ascending auditory pathway: a review.Frontiers in neuroscience · 2023Review
- Is there an unmet medical need for improved hearing restoration?EMBO molecular medicine · 2022Review
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Optogenetic tools, providing non-invasive control over selected cells, have the potential to revolutionize sensory prostheses for humans. Optogenetic stimulation of spiral ganglion neurons (SGNs) in the ear provides a future alternative to electrical stimulation used in cochlear implants. However, most channelrhodopsins do not support the high temporal fidelity pertinent to auditory coding because they require milliseconds to close after light-off. Here, we biophysically characterized the fast channelrhodopsin Chronos and revealed a deactivation time constant of less than a millisecond at body temperature. In order to enhance neural expression, we improved its trafficking to the plasma membrane (Chronos-ES/TS). Following efficient transduction of SGNs using early postnatal injection of the adeno-associated virus AAV-PHPB into the mouse cochlea, fiber-based optical stimulation elicited optical auditory brainstem responses (oABR) with minimal latencies of 1 ms, thresholds of 5 μJ and 100 μs per pulse, and sizable amplitudes even at 1,000 Hz of stimulation. Recordings from single SGNs demonstrated good temporal precision of light-evoked spiking. In conclusion, efficient virus-mediated expression of targeting-optimized Chronos-ES/TS achieves ultrafast optogenetic control of neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.