ArticleCurrent protocols in cell biology2019
Single Molecule RNA FISH (smFISH) in Whole-Mount Mouse Embryonic Organs.
Article in Current protocols in cell biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Post-transcriptional regulatory networks: The dynamic interplay of RNA-binding proteins.The FEBS journal · 2026Review
- Expression of clock genes tracks daily and tidal time in brains of intertidal crustaceans Eurydice pulchra and Parhyale hawaiensis.Current biology : CB · 2025Article
- Protocol for single-molecule FISH in the developing mouse retinal vasculature.STAR protocols · 2024Article
- Microtubule-dependent apical polarization of basement membrane matrix mRNAs in mouse epithelial cells.Cells & development · 2024Article
- Improved enzymatic labeling of fluorescent in situ hybridization probes applied to the visualization of retained introns in cells.RNA (New York, N.Y.) · 2023Article
- Efficient Gene Knockout in Salivary Gland Epithelial Explant Cultures.Journal of dental research · 2023Article
- Application of spatial transcriptome technologies to neurological diseases.Frontiers in cell and developmental biology · 2023Review
- Modeling zero inflation is not necessary for spatial transcriptomics.Genome biology · 2022Article
- Sensitive visualization of SARS-CoV-2 RNA with CoronaFISH.Life science alliance · 2022Article
- Article
- Single-Cell RNA-Sequencing From Mouse Incisor Reveals Dental Epithelial Cell-Type Specific Genes.Frontiers in cell and developmental biology · 2020Article
- Western Blotting with Solutions containing Nanoliter Volumes of Antibody.Current protocols in cell biology · 2019Article
Corrections and comments
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Authors and funding
1 author.
Funding
Abstract
Single molecule RNA fluorescence in situ hybridization (smFISH) has become the standard tool for high spatial resolution analysis of gene expression in the context of tissue organization. This article describes protocols to perform smFISH on whole-mount mouse embryonic organs, where tissue organization can be compared to RNA expression by co-immunostaining of known protein markers. An enzymatic labeling strategy is also introduced to produce low-cost smFISH probes. Important considerations and practical guidelines for imaging smFISH samples using fluorescence confocal microscopy are described. Finally, a suite of custom-written ImageJ macros is included with detailed instructions to enable semi-automated smFISH image analysis of both 2D and 3D images. © 2018 by John Wiley & Sons, Inc.
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Registered trials
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