ArticleMicrobial biotechnology2019
CRISPR/Cas-based screening of a gene activation library in Saccharomyces cerevisiae identifies a crucial role of OLE1 in thermotolerance.
Article in Microbial biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 55 citations in OpenAlex.
- Alterations in lipid saturation trigger remodeling of the outer mitochondrial membrane.Molecular biology of the cell · 2025Article
- Synthetic evolution ofmLife · 2025Review
- The rise and future of CRISPR-based approaches for high-throughput genomics.FEMS microbiology reviews · 2024Review
- Identification of genes associated with the high-temperature fermentation trait in the Saccharomyces cerevisiae natural isolate BCC39850.Archives of microbiology · 2024Article
- Adaptive laboratory evolution under acetic acid stress enhances the multistress tolerance and ethanol production efficiency of Pichia kudriavzevii from lignocellulosic biomass.Scientific reports · 2023Article
- Practical Approaches for the YeastInternational journal of molecular sciences · 2023Review
- Recent Advances in CRISPR-Cas Technologies for Synthetic Biology.Journal of microbiology (Seoul, Korea) · 2023Review
- Exogenous Regulators Enhance the Yield and Stress Resistance of Chlamydospores of the Biocontrol AgentJournal of fungi (Basel, Switzerland) · 2022Article
- Response and regulatory mechanisms of heat resistance in pathogenic fungi.Applied microbiology and biotechnology · 2022Review
- Recent Advances in Directed Yeast Genome Evolution.Journal of fungi (Basel, Switzerland) · 2022Review
- Applications of CRISPR/Cas gene-editing technology in yeast and fungi.Archives of microbiology · 2021Article
- Salt stress improves thermotolerance and high-temperature bioethanol production of multi-stress-tolerant Pichia kudriavzevii by stimulating intracellular metabolism and inhibiting oxidative damage.Biotechnology for biofuels · 2021Article
- Bioprospecting of microbial strains for biofuel production: metabolic engineering, applications, and challenges.Biotechnology for biofuels · 2021Review
- Dual sgRNA-directed gene deletion in basidiomycete Ganoderma lucidum using the CRISPR/Cas9 system.Microbial biotechnology · 2020Article
- CRISPR/Cas9 Systems for the Development ofFrontiers in bioengineering and biotechnology · 2020Review
- Development and Application of CRISPR/Cas in Microbial Biotechnology.Frontiers in bioengineering and biotechnology · 2020Review
- CRISPR/Cas-based screening of a gene activation library in Saccharomyces cerevisiae identifies a crucial role of OLE1 in thermotolerance.Microbial biotechnology · 2019Article
- DNA variants affecting the expression of numerous genes in trans have diverse mechanisms of action and evolutionary histories.PLoS genetics · 2019Article
- CRISPR system in the yeast Saccharomyces cerevisiae and its application in the bioproduction of useful chemicals.World journal of microbiology & biotechnology · 2019Review
- Proteomic profiling and integrated analysis with transcriptomic data bring new insights in the stress responses ofBiotechnology for biofuels · 2019Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRISPR/Cas-based (clustered regularly interspaced short palindromic repeats/CRISPR-associated) screening has been proved to be an efficient method to study functional genomics from yeast to human. In this study, we report the development of a focused CRISPR/Cas-based gene activation library in Saccharomyces cerevisiae and its application in gene identification based on functional screening towards improved thermotolerance. The gene activation library was subjected to screening at 42°C, and the same library cultured at 30°C was set as a control group. After five successive subcultures, five clones were randomly picked from the libraries cultured at 30 and 42°C, respectively. The five clones selected at 30°C contain the specificity sequences of five different single guide RNAs, whereas all the five clones selected at 42°C contain the specificity sequence of one sgRNA that targets the promoter region of OLE1. A crucial role of OLE1 in thermotolerance was identified: the overexpression of OLE1 increased fatty acid unsaturation, and thereby helped counter lipid peroxidation caused by heat stress, rendering the yeast thermotolerant. This study described the application of CRISPR/Cas-based gene activation screening with an example of thermotolerant yeast screening, demonstrating that this method can be used to identify functional genes in yeast.
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