Evidence map›Paper›PMID 30394398›Full record

ArticleJournal of visualized experiments : JoVE2018

Gene Expression Analysis of Endothelial Cells Exposed to Shear Stress Using Multiple Parallel-plate Flow Chambers.

H S Jeffrey Man, Aravin N Sukumar, Kyung Ha Ku, Michelle K Dubinsky, Noeline Subramaniam, Philip A Marsden

Open access · greenAbstract readVideo-Audio Media
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

H S Jeffrey ManInstitute of Medical Science, University of Toronto; Keenan Research Centre in the Li Ka Shing Knowledge Institute, St. Michael's Hospital.
Aravin N SukumarInstitute of Medical Science, University of Toronto; Keenan Research Centre in the Li Ka Shing Knowledge Institute, St. Michael's Hospital.
Kyung Ha KuKeenan Research Centre in the Li Ka Shing Knowledge Institute, St. Michael's Hospital; Department of Laboratory Medicine and Pathobiology, University of Toronto.
Michelle K DubinskyInstitute of Medical Science, University of Toronto; Keenan Research Centre in the Li Ka Shing Knowledge Institute, St. Michael's Hospital.
Noeline SubramaniamInstitute of Medical Science, University of Toronto; Keenan Research Centre in the Li Ka Shing Knowledge Institute, St. Michael's Hospital.
Philip A MarsdenInstitute of Medical Science, University of Toronto; Keenan Research Centre in the Li Ka Shing Knowledge Institute, St. Michael's Hospital; Department of Laboratory Medicine and Pathobiology, University of Toronto; Department of Medicine, University of Toronto; p.marsden@utoronto.ca.
St. Michael's Hospital · CAUniversity of Toronto · CA

Funding

CIHR
6 · The paper itself

Abstract

We describe a workflow for the analysis of gene expression from endothelial cells subject to a steady laminar flow using multiple monitored parallel-plate flow chambers. Endothelial cells form the inner cellular lining of blood vessels and are chronically exposed to the frictional force of blood flow called shear stress. Under physiological conditions, endothelial cells function in the presence of various shear stress conditions. Thus, the application of shear stress conditions in in vitro models can provide greater insight into endothelial responses in vivo. The parallel-plate flow chamber previously published by Lane et al.

Indexed as

Cells, CulturedEndothelial CellsGene ExpressionGene Expression RegulationHemodynamicsHumansStress, Mechanical

Identifiers

PMID30394398
PMCPMC6235583
OpenAlexW2897482365

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.