ArticleJournal of visualized experiments : JoVE2018
Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
47 citing papers in PubMed.
- Association of Monocyte Subsets with Carotid Artery Stenosis Severity.Medicina (Kaunas, Lithuania) · 2026Article
- Monocytes Display Altered Inflammatory Profiles in Individuals with Diabetic Foot Ulcers Indicating Monocyte Dysfunction.European journal of immunology · 2026Article
- CD300e modulates metabolic programs in adipose tissue macrophages during obesity.Cell death & disease · 2026Article
- Peripheral Blood Cells and Clinical Profiles as Biomarkers for Pain Detection in Palliative Care Patients.Biomedicines · 2026Article
- "Monocytes in B-Cell Malignancies: Their Role in Disease Progression and Therapy Resistance".Current oncology reports · 2025Review
- Monocytes from people with Familial Hypercholesterolaemia are inflammatory, despite statin-treatment.Atherosclerosis plus · 2025Article
- Monocyte-macrophage dynamics as key in disparate lung and peripheral immune responses in severe anti-melanoma differentiation-associated gene 5-positive dermatomyositis-related interstitial lung disease.Clinical and translational medicine · 2025Article
- The molecular interplay among gut dysbiosis, adipose tissue, and metabolite-derived damage-associated molecular patterns in metainflammation and atherogenesis.Frontiers in immunology · 2025Review
- Human Papillomavirus Vaccine immune responses in an Olive baboon model is not compromised by chronicAIMS microbiology · 2025Article
- Human Blood Non-Classical/Classical Monocyte Cells Are Heterogeneously Presented in Severe COVID-19 and Correlate with Disease Activity.Journal of innate immunity · 2025Article
- Changes in immune cell populations during acclimatization to high altitude.Physiological reports · 2024Article
- Medial prefrontal cortex connectivity with the nucleus accumbens is related to HIV serostatus, perceptions of psychological stress, and monocyte expression of TNF-a.Brain, behavior, & immunity - health · 2024Article
- Design of a Robust Flow Cytometric Approach for Phenotypical and Functional Analysis of Human Monocyte Subsets in Health and Disease.Biomolecules · 2024Article
- CD74 is a functional MIF receptor on activated CD4Cellular and molecular life sciences : CMLS · 2024Article
- Transcriptomics analysis highlights potential ways in human pathogenesis in Leishmania braziliensis infected with the viral endosymbiont LRV1.PLoS neglected tropical diseases · 2024Article
- A sulfonimide derivative of bezafibrate as a dual inhibitor of cyclooxygenase-2 and PPARα.Frontiers in pharmacology · 2024Article
- Markers of immunosenescence in CMV seropositive healthy elderly adults.Frontiers in aging · 2024Article
- Expression of CD40 and CD192 in Classical Monocytes in Multiple Sclerosis Patients Assessed with Transcranial Magnetic Stimulation.Biomedicines · 2023Article
- Monocytes in the Characterization of Pain in Palliative Patients with Severe Dementia-A Pilot Study.International journal of molecular sciences · 2023Article
- Monocyte Differentiation and Heterogeneity: Inter-Subset and Interindividual Differences.International journal of molecular sciences · 2023Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Monocytes are key contributors in various inflammatory disorders and alterations to these cells, including their subset proportions and functions, can have pathological significance. An ideal method for examining alterations to monocytes is whole blood flow cytometry as the minimal handling of samples by this method limits artifactual cell activation. However, many different approaches are taken to gate the monocyte subsets leading to inconsistent identification of the subsets between studies. Here we demonstrate a method using whole blood flow cytometry to identify and characterize human monocyte subsets (classical, intermediate, and non-classical). We outline how to prepare the blood samples for flow cytometry, gate the subsets (ensure contaminating cells have been removed), and determine monocyte subset expression of surface markers - in this example M1 and M2 markers. This protocol can be extended to other studies that require a standard gating method for assessing monocyte subset proportions and monocyte subset expression of other functional markers.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.