ArticleMicromachines2018
Analysis of PCR Kinetics inside a Microfluidic DNA Amplification System.
Article in Micromachines, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Structure Optimization of Polymerase Chain Reaction Devices Under High Flow Rate: A Numerical Study.Micromachines · 2025Article
- Assessing Different PCR Master Mixes for Ultrarapid DNA Amplification: Important Analytical Parameters.Diagnostics (Basel, Switzerland) · 2024Article
- Low-Temperature Loop-Mediated Isothermal Amplification Operating at Physiological Temperature.Biosensors · 2023Article
- Detection Method for Tomato Leaf Mildew Based on Hyperspectral Fusion Terahertz Technology.Foods (Basel, Switzerland) · 2023Article
- Kinetics of elementary steps in loop-mediated isothermal amplification (LAMP) show that strand invasion during initiation is rate-limiting.Nucleic acids research · 2023Article
- Application of Microfluidics for Bacterial Identification.Pharmaceuticals (Basel, Switzerland) · 2022Review
- A Portable Continuous-Flow Polymerase Chain Reaction Chip Device Integrated with Arduino Boards for DetectingMicromachines · 2022Article
- A Rapid Detection Method for Fungal Spores from Greenhouse Crops Based on CMOS Image Sensors and Diffraction Fingerprint Feature Processing.Journal of fungi (Basel, Switzerland) · 2022Article
- Integrated microdevice with a windmill-like hole array for the clog-free, efficient, and self-mixing enrichment of circulating tumor cells.Microsystems & nanoengineering · 2022Article
- A Rapid Detection Method for Tomato Gray Mold Spores in Greenhouse Based on Microfluidic Chip Enrichment and Lens-Less Diffraction Image Processing.Foods (Basel, Switzerland) · 2021Article
- Portable Rice Disease Spores Capture and Detection Method Using Diffraction Fingerprints on Microfluidic Chip.Micromachines · 2019Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to analyze the DNA amplification numerically with integration of the DNA kinetics, three-dimensional simulations, including flow and thermal fields, and one-dimensional polymerase chain reaction (PCR) kinetics are presented. The simulated results are compared with experimental data that have been applied to the operation of a continuous-flow PCR device. Microchannels fabricated by Micro Electro-Mechanical Systems (MEMS) technologies are shown. Comprehensive simulations of the flow and thermal fields and experiments measuring temperatures during thermal cycling are presented first. The resultant velocity and temperature profiles from the simulations are introduced to the mathematical models of PCR kinetics. Then kinetic equations are utilized to determine the evolution of the species concentrations inside the DNA mixture along the microchannel. The exponential growth of the double-stranded DNA concentration is investigated numerically with the various operational parameters during PCR. Next a 190-bp segment of
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