ArticleBiomaterials2019
The role of chondroitin sulfate in regulating hypertrophy during MSC chondrogenesis in a cartilage mimetic hydrogel under dynamic loading.
Article in Biomaterials, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 91 citations in OpenAlex.
- Article
- Injectable polysaccharide hydrogel microspheres integrated with BPNS-ZnO nanocomposites restore redox homeostasis and protect cartilage in osteoarthritis.Materials today. Bio · 2026Article
- Sulfated polysaccharide reprograms arterialized angiogenesis of endochondral ossification to rescue defective osteonecrosis therapy.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Zonally patterned demineralized bone matrix-meniscus ECM composite scaffold directing region-specific fibrochondrogenic and angiogenic responses.Regenerative biomaterials · 2026Article
- Lacc1-engineered extracellular vesicles reprogram mitochondrial metabolism to alleviate inflammation and cartilage degeneration in TMJ osteoarthritis.Journal of nanobiotechnology · 2025Article
- Ultramodern natural and synthetic polymer hydrogel scaffolds for articular cartilage repair and regeneration.Biomedical engineering online · 2025Review
- Polysaccharide-based biomaterials for regenerative therapy in intervertebral disc degeneration.Materials today. Bio · 2025Review
- Differentiation of stem cells into chondrocytes and their potential clinical application in cartilage regeneration.Histochemistry and cell biology · 2025Review
- Cell-recruited microspheres for OA treatment by dual-modulating inflammatory and chondrocyte metabolism.Materials today. Bio · 2024Article
- Intra-Articular Injectable Biomaterials for Cartilage Repair and Regeneration.Advanced healthcare materials · 2024Review
- Chondroitin Sulfate/Hyaluronic Acid-Blended Hydrogels Suppress Chondrocyte Inflammation under Pro-Inflammatory Conditions.ACS biomaterials science & engineering · 2024Article
- Collagen Type II-Based Injectable Materials for In situ Repair and Regeneration of Articular Cartilage Defect.Biomaterials research · 2024Article
- Identification of candidate genes and chemicals associated with osteonecrosis of femoral head by multiomics studies and chemical-gene interaction analysis.Frontiers in endocrinology · 2024Article
- Cell unit-inspired natural nano-based biomaterials as versatile building blocks for bone/cartilage regeneration.Journal of nanobiotechnology · 2023Review
- Fabricating the cartilage: recent achievements.Cytotechnology · 2023Review
- Click and bioorthogonal hyaluronic acid hydrogels as an ultra-tunable platform for the investigation of cell-material interactions.Bioactive materials · 2023Article
- The Effects of Mechanical Load on Chondrogenic Responses of Bone Marrow Mesenchymal Stem Cells and Chondrocytes Encapsulated in Chondroitin Sulfate-Based Hydrogel.International journal of molecular sciences · 2023Article
- Nanoengineered hydrogels as 3D biomimetic extracellular matrix with injectable and sustained delivery capability for cartilage regeneration.Bioactive materials · 2023Article
- Review
- A 3D printed mimetic composite for the treatment of growth plate injuries in a rabbit model.NPJ Regenerative medicine · 2022Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Mesenchymal stem cells (MSCs) are promising for cartilage regeneration, but readily undergo terminal differentiation. The aim of this study was two-fold: a) investigate physiochemical cues from a cartilage-mimetic hydrogel under dynamic compressive loading on MSC chondrogenesis and hypertrophy and b) identify whether Smad signaling and p38 MAPK signaling mediate hypertrophy during MSC chondrogenesis. Human MSCs were encapsulated in photoclickable poly(ethylene glycol) hydrogels containing chondroitin sulfate and RGD, cultured under dynamic compressive loading or free swelling for three weeks, and evaluated by qPCR and immunohistochemistry. Loading inhibited hypertrophy in the cartilage-mimetic hydrogel indicated by a reduction in pSmad 1/5/8, Runx2, and collagen X proteins, while maintaining chondrogenesis by pSmad 2/3 and collagen II proteins. Inhibiting pSmad 1/5/8 under free swelling culture significantly reduced collagen X protein, similar to the loading condition. Chondroitin sulfate was necessary for load-inhibited hypertrophy and correlated with enhanced S100A4 expression, which is downstream of the osmotic responsive transcription factor NFAT5. Inhibiting p38 MAPK under loading reduced S100A4 expression, and upregulated Runx2 and collagen X protein. Findings from this study indicate that chondroitin sulfate with dynamic loading create physiochemical cues that support MSC chondrogenesis and attenuate hypertrophy through Smad 1/5/8 inhibition and p38 MAPK upregulation.
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