Evidence map›Paper›PMID 30391630›Full record

ArticleThe Journal of infection2018

Genomes of Escherichia coli bacteraemia isolates originating from urinary tract foci contain more virulence-associated genes than those from non-urinary foci and neutropaenic hosts.

Adam P Dale, Anish K Pandey, Richard J Hesp, Konstantinos Belogiannis, Jay R Laver, Clifford C Shone, Robert C Read

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of infection, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Urinary Tract Infections Caused byFrontiers in cellular and infection microbiology · 2022
    Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Diversity of Virulence Genes in Multidrug ResistantInfection and drug resistance · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Adam P DaleAcademic Unit of Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, LC71A, C Level, South Academic Block, Southampton General Hospital, Southampton SO16 6YD, UK. Electronic address: a.p.dale@soton.ac.uk.
Anish K PandeyAcademic Unit of Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, LC71A, C Level, South Academic Block, Southampton General Hospital, Southampton SO16 6YD, UK.
Richard J HespNational Infection Service, Public Health England, Porton Down, Salisbury SP4 0JG, UK.
Konstantinos BelogiannisAcademic Unit of Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, LC71A, C Level, South Academic Block, Southampton General Hospital, Southampton SO16 6YD, UK.
Jay R LaverAcademic Unit of Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, LC71A, C Level, South Academic Block, Southampton General Hospital, Southampton SO16 6YD, UK.
Clifford C ShoneNational Infection Service, Public Health England, Porton Down, Salisbury SP4 0JG, UK.
Robert C ReadAcademic Unit of Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, LC71A, C Level, South Academic Block, Southampton General Hospital, Southampton SO16 6YD, UK; NIHR Southampton Biomedical Research Centre, University Hospital Southampton, Southampton SO16 6YD, UK.
Southampton General Hospital · GBPublic Health England · GB

Funding

Department of Health NF-SI-0617-10010Medical Research Council MR/N013204/1Medical Research Council MR/N026993/1Wellcome Trust 203581/Z/16/Z
6 · The paper itself

Abstract

objectivesEscherichia coli is the leading cause of bacteraemia. In an era of emerging multi-drug-resistant strains, development of effective preventative strategies will be informed by knowledge of strain diversity associated with specific infective syndromes/patient groups. We hypothesised that the number of virulence factor (VF) genes amongst bacteraemia isolates from neutropaenic patients would be lower than isolates from immunocompetent patients.

methodsImmunocompetent and neutropaenic adults with E. coli bacteraemia were recruited prospectively and the source of bacteraemia determined. VF gene profiles were established in silico following whole genome sequencing.

resultsIsolates from individual patients were monoclonal. Strains from immunocompetent patients with urinary tract infective foci (UTIF) harboured more VF genes (median number of VF genes 16, range 8-24) than isolates from both immunocompetent patients with non-UTIF (10, 2-22, p = 0.0058) and neutropaenic patients with unknown focus of infection (NPUFI) (8, 3-13, p < 0.0001). Number of VF genes (OR 1.21, 95% CIs 1.01-1.46, p = 0.039) and urinary catheter/recurrent urinary tract infection (OR 12.82, 95% CIs 1.24-132.65, p = 0.032) were independent predictors of bacteraemia secondary to UTIF vs. non-UTIF in immunocompetent patients. papA, papC, papE/F, papG, agn43, tia, iut, fyuA, kpsM and sat were significantly more prevalent amongst UTIF- vs non-UTIF-originating isolates amongst immunocompetent patients, while papC, papE/F, papG, agn43, tia, fyuA, hlyA, usp and clb were significantly more prevalent amongst UTIF- vs NPUFI-associated isolates.

conclusionsBacteraemia-associated E. coli strains originating from UTIF have distinct VF gene profiles from strains associated with non-UTIF- and NPUFI. This diversity must be addressed in the design of future vaccines to ensure adequate coverage of strains responsible for site-specific disease.

Indexed as

Genome, BacterialAdultAgedAged, 80 and overBacteremiaEscherichia coliEscherichia coli InfectionsFemaleHumansImmunocompetenceMaleMiddle AgedNeutropeniaPhylogenyProspective StudiesSequence Analysis, DNAVirulence FactorsAntimicrobial resistanceBacteraemiaEscherichia coliExPECNeutropaeniaVirulence factorWhole genome sequencing

Identifiers

PMID30391630
PMCPMC6293314
OpenAlexW2898928315

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.