ArticleCell systems2018
Unbiased Fitness Estimation of Pooled Barcode or Amplicon Sequencing Studies.
Article in Cell systems, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Direct estimation of genotype fitness from time series.bioRxiv : the preprint server for biology · 2026Article
- Putting BASIL in a BLT: A Bayesian filtering method for estimating the fitness effects of nascent adaptive mutations.PLoS computational biology · 2026Article
- Experimental evolution in an era of molecular manipulation.Nature reviews. Genetics · 2026Review
- Global epistasis in budding yeast driven by many natural variants whose effects scale with fitness.Genetics · 2025Article
- Doblin: inferring dominant clonal lineages from high-resolution DNA barcoding time series.Bioinformatics (Oxford, England) · 2025Article
- Variant scoring tools for deep mutational scanning.Molecular systems biology · 2025Review
- Precise lineage tracking using molecular barcodes demonstrates fitness trade-offs for ivermectin resistance in nematodes.G3 (Bethesda, Md.) · 2025Article
- Saturated Transposon Analysis in Yeast as a one-step method to quantify the fitness effects of gene disruptions on a genome-wide scale.PloS one · 2025Article
- Pooled PPIseq: Screening the SARS-CoV-2 and human interface with a scalable multiplexed protein-protein interaction assay platform.PloS one · 2025Article
- Distinguishing mutants that resist drugs via different mechanisms by examining fitness tradeoffs.eLife · 2024Article
- Distinguishing mutants that resist drugs via different mechanisms by examining fitness tradeoffs.bioRxiv : the preprint server for biology · 2024Article
- Genome-scale analysis of interactions between genetic perturbations and natural variation.Nature communications · 2024Article
- Genome-scale analysis of interactions between genetic perturbations and natural variation.bioRxiv : the preprint server for biology · 2024Article
- Fit-Seq2.0: An Improved Software for High-Throughput Fitness Measurements Using Pooled Competition Assays.Journal of molecular evolution · 2023Article
- Extreme Sensitivity of Fitness to Environmental Conditions: Lessons from #1BigBatch.Journal of molecular evolution · 2023Article
- Resolving Deleterious and Near-Neutral Effects Requires Different Pooled Fitness Assay Designs.Journal of molecular evolution · 2023Article
- Intra-Frontiers in genetics · 2023Article
- Random peptides rich in small and disorder-promoting amino acids are less likely to be harmful.Genome biology and evolution · 2022Article
- The interplay of additivity, dominance, and epistasis on fitness in a diploid yeast cross.Nature communications · 2022Article
- The Effects of Sequence Length and Composition of Random Sequence Peptides on the Growth ofGenes · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Standard practice for phenotyping complex cell pools is to measure the fold enrichment of genotype-specific amplicons after a period of competitive growth. Here, we show that fold-enrichment measures cannot be compared across genotype pools with different fitness distributions. We develop a method to calculate an unbiased estimate of relative fitness by tracking abundances over several time points and show how to optimize experimental protocols to minimize fitness measurement error.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.