ArticleNature protocols2018
CRISPR-Cas9-mediated genome editing in apple and grapevine.
Article in Nature protocols, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
86 citing papers in PubMed, 1 synthesis or guideline pooled it, 204 citations in OpenAlex.
- Chickpea Biofortification for Cytokinin DehydrogenaseFrontiers in genetics · 2022Pooled it
- Molecular insights and integrated management of Glomerella leaf spot in apple.Horticulture research · 2026Review
- Precision editing to improve fruit traits: CRISPR/Cas into the picture.Protoplasma · 2026Review
- Gene Flow and Hybridization Potential Between GM/NGT Crops and Conventional Varieties or Wild Relatives: A Scoping Literature Review with Emphasis on Oilseed Rape (Biotech (Basel (Switzerland)) · 2026Review
- Molecular insights into the regulation of flavonoid biosynthesis in fruits.Horticulture research · 2026Article
- Root-Specific Signal Modules Mediating Abiotic Stress Tolerance in Fruit Crops.Plants (Basel, Switzerland) · 2026Review
- Tiny but powerful: protoplast isolation inFrontiers in plant science · 2026Article
- Integrative genomics for mango genetics and breeding.Horticulture research · 2026Article
- A voyage of reprogrammable metabolic bioengineering reshapes plant defense: from editing tools to synthetic systems.Frontiers in plant science · 2026Review
- In Vitro Regeneration of Southern Italian Grapevine Cultivars from Embryogenic Calluses and Protoplasts.Plants (Basel, Switzerland) · 2025Article
- CRISPR/Cas system-mediated transgene-free or DNA-free genome editing in plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
- CRISPR/Cas9-mediated editing of carotenoid biosynthesis genes alters carotenoid concentrations in kiwifruit.BMC plant biology · 2025Article
- The MdHB7L-MdICE1L-MdHOS1 Module Fine-Tunes Apple Cold Response via CBF-Dependent and CBF-Independent Pathways.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Transgene-free Genome Editing in Grapevine.Bio-protocol · 2025Article
- Advances in the molecular mechanism of grapevine resistance to fungal diseases.Molecular horticulture · 2025Review
- Unlocking male sterility in horticultural crops through gene editing technology for precision breeding applications: presentation of a case study in tomato.Frontiers in plant science · 2025Article
- Mini review: Apple improvement, traditional approaches, biotechnology options, and regulatory considerations.Frontiers in bioengineering and biotechnology · 2025Review
- A New Approach for CRISPR/Cas9 Editing and Selection of Pathogen-Resistant Plant Cells of Wine Grape cv. 'Merlot'.International journal of molecular sciences · 2024Article
- Impacts of Climate Change and Mitigation Strategies for Some Abiotic and Biotic Constraints Influencing Fruit Growth and Quality.Plants (Basel, Switzerland) · 2024Review
- New improvements in grapevine genome editing: high efficiency biallelic homozygous knock-out from regenerated plantlets by using an optimized zCas9i.Plant methods · 2024Article
26 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 8 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The CRISPR-Cas9 genome-editing tool and the availability of whole-genome sequences from plant species have revolutionized our ability to introduce targeted mutations into important crop plants, both to explore genetic changes and to introduce new functionalities. Here, we describe protocols adapting the CRISPR-Cas9 system to apple and grapevine plants, using both plasmid-mediated genome editing and the direct delivery of CRISPR-Cas9 ribonucleoproteins (RNPs) to achieve efficient DNA-free targeted mutations in apple and grapevine protoplasts. We provide a stepwise protocol for the design and transfer of CRISPR-Cas9 components to apple and grapevine protoplasts, followed by verification of highly efficient targeted mutagenesis, and regeneration of plants following the plasmid-mediated delivery of components. Our plasmid-mediated procedure and the direct delivery of CRISPR-Cas9 RNPs can both be utilized to modulate traits of interest with high accuracy and efficiency in apple and grapevine, and could be extended to other crop species. The complete protocol employing the direct delivery of CRISPR-Cas9 RNPs takes as little as 2-3 weeks, whereas the plasmid-mediated procedure takes >3 months to regenerate plants and study the mutations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.