ArticleScientific reports2018
Isotopic resonance at 370 ppm deuterium negatively affects kinetics of luciferin oxidation by luciferase.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 12 citations in OpenAlex.
- Deuterium-depleted water modulates lipid metabolism in the freshwater microalga Chlorella sp.World journal of microbiology & biotechnology · 2026Article
- Slight Deuterium Enrichment in Water Acts as an Antioxidant: Is Deuterium a Cell Growth Regulator?Molecular & cellular proteomics : MCP · 2020Article
- Dependence of Biocatalysis on D/H Ratio: Possible Fundamental Differences for High-Level Biological Taxons.Molecules (Basel, Switzerland) · 2020Article
- Possible Mechanisms of Biological Effects Observed in Living Systems duringMolecules (Basel, Switzerland) · 2019Review
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since 1930s, it has been known that some biochemical and biological processes exhibit abnormal kinetics at a deuterium concentration in the local environment of 250-600 ppm, which is 2-4 times higher that the normal concentration of 150 ppm D. We sought to test if the kinetics of firefly luciferase oxidizing luciferin, the reaction widely used as a read-out in various biochemical assays, is also affected by an elevated deuterium content. To this end, both luciferase and luciferin substrate solutions were prepared based on water with extra deuterium added to a concentration ranging from 150 ppm and up to 10,000 ppm (1%). Upon mixing the solutions, the luminescence intensity at different times was compared with that of the corresponding control solutions with 150 ppm D. A broad negative resonance was detected (p < 10
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