ArticleScientific reports2018
Fast Optical Sectioning for Widefield Fluorescence Mesoscopy with the Mesolens based on HiLo Microscopy.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Mesolens imaging in microbiology.Essays in biochemistry · 2026Review
- Exploring pancreatic beta-cell subgroups and their connectivity.Nature metabolism · 2024Review
- Time-lapse mesoscopy ofMicrobiology (Reading, England) · 2024Article
- Intra-colony channel morphology inBiofilm · 2022Article
- Light-sheet mesoscopy with the Mesolens provides fast sub-cellular resolution imaging throughout large tissue volumes.iScience · 2022Article
- Challenges and advances in optical 3D mesoscale imaging.Journal of microscopy · 2022Article
- Application of Light-Sheet Mesoscopy to Image Host-Pathogen Interactions in Intact Organs.Frontiers in cellular and infection microbiology · 2022Article
- Optical mesoscopy, machine learning, and computational microscopy enable high information content diagnostic imaging of blood films.The Journal of pathology · 2021Article
- Intra-colony channels in E. coli function as a nutrient uptake system.The ISME journal · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
We present here a fast optical sectioning method for mesoscopy based on HiLo microscopy, which makes possible imaging of specimens of up to 4.4 mm × 3 mm × 3 mm in volume in under 17 hours (estimated for a z-stack comprising 1000 images excluding computation time) with subcellular resolution throughout. Widefield epifluorescence imaging is performed with the Mesolens using a high pixel-number camera capable of sensor-shifting to generate a 259.5 Megapixel image, and we have developed custom software to perform HiLo processing of the very large datasets. Using this method, we obtain comparable sectioning strength to confocal laser scanning microscopy (CLSM), with sections as thin as 6.8 ± 0.2 μm and raw acquisition speed of 1 minute per slice which is up to 30 times faster than CLSM on the full field of view (FOV) of the Mesolens of 4.4 mm with lateral resolution of 0.7 μm and axial resolution of 7 μm. We have applied this HiLo mesoscopy method to image fixed and fluorescently stained hippocampal neuronal specimens and a 5-day old zebrafish larva.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.