ArticleScientific reports2018
Fast confocal fluorescence imaging in freely behaving mice.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- Miniscope Zero: a fully wireless, single-cell-resolution miniature microscope for imaging neural dynamics in freely behaving animals.bioRxiv : the preprint server for biology · 2026Article
- Quantifying optical sectioning in reflection microscopy with patterned illumination.Biomedical optics express · 2026Article
- Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits.ACS photonics · 2025Review
- Integrating Optics and Parametrically-Resonant Micro-Scanner Design for Large Working Distance Implantable Microscopy.IEEE/ASME transactions on mechatronics : a joint publication of the IEEE Industrial Electronics Society and the ASME Dynamic Systems and Control Division · 2025Article
- Wide-field fluorescence navigation system for efficient miniature multiphoton imaging in freely behaving animals.Neurophotonics · 2025Article
- Semi-automated analysis of cerebral capillary red blood cell velocities allows modeling of transit time distribution after experimental subarachnoid hemorrhage in mice.Neurophotonics · 2025Article
- The Role of Virtual Reality and Artificial Intelligence in Cognitive Pain Therapy: A Narrative Review.Current pain and headache reports · 2024Review
- Recent advances in light patterned optogenetic photostimulation in freely moving mice.Neurophotonics · 2024Article
- Imaging the brain in action: a motorized optical rotary joint for wide field fibroscopy in freely moving animals.Neurophotonics · 2023Article
- Advances of optical miniscopes forFrontiers in neuroscience · 2022Review
- Emerging imaging methods to study whole-brain function in rodent models.Translational psychiatry · 2021Review
- Two-Photon Fluorescence Imaging.Advances in experimental medicine and biology · 2021Article
- Miniature Fluorescence Microscopy for Imaging Brain Activity in Freely-Behaving Animals.Neuroscience bulletin · 2020Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence imaging in the brain of freely behaving mice is challenging due to severe miniaturization constraints. In particular, the ability to image a large field of view at high temporal resolution and with efficient out-of-focus background rejection still raises technical difficulties. Here, we present a novel fiberscope system that provides fast (up to 200 Hz) background-free fluorescence imaging in freely behaving mice over a field of view of diameter 230 μm. The fiberscope is composed of a custom-made multipoint-scanning confocal microscope coupled to the animal with an image guide and a micro-objective. By simultaneously registering a multipoint-scanning confocal image and a conventional widefield image, we subtracted the residual out-of-focus background and provided a background-free confocal image. Illumination and detection pinholes were created using a digital micromirror device, providing high adaptability to the sample structure and imaging conditions. Using this novel imaging tool, we demonstrated fast fluorescence imaging of microvasculature up to 120 μm deep in the mouse cortex, with an out-of-focus background reduced by two orders of magnitude compared with widefield microscopy. Taking advantage of the high acquisition rate (200 Hz), we measured red blood cell velocity in the cortical microvasculature and showed an increase in awake, unrestrained mice compared with anaesthetized animals.
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