ArticleScientific reports2018
Combining morpho-taxonomy and metabarcoding enhances the detection of non-indigenous marine pests in biofouling communities.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Spatio-temporal metabarcoding surveys in ports reveal homogenised communities of non-indigenous species with high genetic diversity and connectivity.Scientific reports · 2026Article
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- Environmental implications of future offshore renewable energy development in Aotearoa New Zealand.Journal of the Royal Society of New Zealand · 2025Review
- A new sampling device for metabarcoding surveillance of port communities and detection of non-indigenous species.iScience · 2024Article
- VESPA: an optimized protocol for accurate metabarcoding-based characterization of vertebrate eukaryotic endosymbiont and parasite assemblages.Nature communications · 2024Article
- Harnessing environmental DNA to reveal biogeographical patterns of non-indigenous species for improved co-governance of the marine environment in Aotearoa New Zealand.Scientific reports · 2023Article
- Marine Flora of French Polynesia: An Updated List Using DNA Barcoding and Traditional Approaches.Biology · 2023Article
- Microbial community shift on artificial biological reef structures (ABRs) deployed in the South China Sea.Scientific reports · 2023Article
- Biopollution by Invasive Marine Non-Indigenous Species: A Review of Potential Adverse Ecological Effects in a Changing Climate.International journal of environmental research and public health · 2021Review
- eDNA metabarcoding of small plankton samples to detect fish larvae and their preys from Atlantic and Pacific waters.Scientific reports · 2021Article
- Comparison of Larval Fish Detections Using Morphology-Based Taxonomy versus High-Throughput Sequencing for Invasive Species Early Detection.Canadian journal of fisheries and aquatic sciences. Journal canadien des sciences halieutiques et aquatiques · 2021Article
- Investigating variability in microbial community composition in replicate environmental DNA samples down lake sediment cores.PloS one · 2021Article
- The risks of using molecular biodiversity data for incidental detection of species of concern.Diversity & distributions · 2020Article
- Monitoring of biofouling communities in a Portuguese port using a combined morphological and metabarcoding approach.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Marine infrastructure can favor the spread of non-indigenous marine biofouling species by providing a suitable habitat for them to proliferate. Cryptic organisms or those in early life stages can be difficult to distinguish by conventional morphological taxonomy. Molecular tools, such as metabarcoding, may improve their detection. In this study, the ability of morpho-taxonomy and metabarcoding (18S rRNA and COI) using three reference databases (PR2, BOLD and NCBI) to characterize biodiversity and detect non-indigenous species (NIS) in biofouling was compared on 60 passive samplers deployed over summer and winter in a New Zealand marina. Highest resolution of metazoan taxa was identified using 18S rRNA assigned to PR2. There were higher assignment rates to NCBI reference sequences, but poorer taxonomic identification. Using all methods, 48 potential NIS were identified. Metabarcoding detected the largest proportion of those NIS: 77% via 18S rRNA/PR2 and NCBI and 35% via COI/BOLD and NCBI. Morpho-taxonomy detected an additional 14% of all identified NIS comprising mainly of bryozoan taxa. The data highlight several on-going challenges, including: differential marker resolution, primer biases, incomplete sequence reference databases, and variations in bioinformatic pipelines. Combining morpho-taxonomy and molecular analysis methods will likely enhance the detection of NIS from complex biofouling.
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