Evidence map›Paper›PMID 30389828›Full record

ArticleRNA (New York, N.Y.)2019

Carbodiimide reagents for the chemical probing of RNA structure in cells.

Peter Y Wang, Alec N Sexton, William J Culligan, Matthew D Simon

Open access · bronzeAbstract read
In one paragraph

Article in RNA (New York, N.Y.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
2.2field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 60 citations in OpenAlex.

  1. A nucleic acid labeling chemistry reveals surface DNA on exosomes.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  2. Article
  3. Review
  4. A nucleic acid labeling chemistry reveals surface DNA on exosomes.bioRxiv : the preprint server for biology · 2025
    Article
  5. Analysis of the Effects of Sugar Modifications on RNA Chemical Ligation Reactions.Chembiochem : a European journal of chemical biology · 2025
    Article
  6. Efficient Assembly of Functional RNA byJournal of the American Chemical Society · 2025
    Article
  7. Article
  8. RNA Structure: Past, Future, and Gene Therapy Applications.International journal of molecular sciences · 2024
    Review
  9. Article
  10. Article
  11. In vivo structure probing of RNA inRNA (New York, N.Y.) · 2023
    Article
  12. Article
  13. Review
  14. Article
  15. Article
  16. In vivo secondary structural analysis of Influenza A virus genomic RNA.Cellular and molecular life sciences : CMLS · 2023
    Article
  17. Article
  18. Review
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Peter Y WangDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511, USA.
Alec N SextonDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511, USA.
William J CulliganDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511, USA.
Matthew D SimonDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511, USA.
Yale University · US

Funding

MEDICAL SCIENTIST TRAINING PROGRAMT32GM007205 · NIGMS · YALE UNIVERSITY · PI KAZMIERCZAK, BARBARA I · 1985 to 2019
$42.9M
Skill Development for Diverse Scientific Careers: A New Course Predoctoral Program in Cellular and Molecular BiologyT32GM007223 · NIGMS · YALE UNIVERSITY · PI BASERGA, SUSAN J · 1985 to 2019
$31.0M
Integrating RNAs into signaling pathways by engineering covalent RNA modificationDP2HD083992 · NICHD · YALE UNIVERSITY · PI SIMON, MATTHEW DAVID · 2014 to 2014
$2.5M
NICHD NIH HHS DP2 HD083992NIGMS NIH HHS T32 GM007205NIGMS NIH HHS T32 GM007223NIH HHS DP2 HD083992-01NIH HHS T32GM007205NIH HHS T32GM007223
6 · The paper itself

Abstract

Deciphering the conformations of RNAs in their cellular environment allows identification of RNA elements with potentially functional roles within biological contexts. Insight into the conformation of RNA in cells has been achieved using chemical probes that were developed to react specifically with flexible RNA nucleotides, or the Watson-Crick face of single-stranded nucleotides. The most widely used probes are either selective SHAPE (2'-hydroxyl acylation and primer extension) reagents that probe nucleotide flexibility, or dimethyl sulfate (DMS), which probes the base-pairing at adenine and cytosine but is unable to interrogate guanine or uracil. The constitutively charged carbodiimide

Indexed as

CarbodiimidesMolecular ProbesAnimalsBase PairingBase SequenceCells, CulturedIndicators and ReagentsMiceMolecular Probe TechniquesMolecular StructureNucleic Acid ConformationRNARNA, Long NoncodingSulfuric Acid Esters1-ethyl-3-(3-(diethylamino)propyl)carbodiimideCarbodiimidesdimethyl sulfateIndicators and ReagentsMolecular ProbesRNARNA, Long NoncodingSulfuric Acid EstersXIST non-coding RNAcarbodiimidechemical probingin-cellRNA structure

Identifiers

PMID30389828
PMCPMC6298570
OpenAlexW2899250482

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.