ArticleThe Journal of clinical investigation1987
Purification of human erythroid colony-forming units and demonstration of specific binding of erythropoietin.
Article in The Journal of clinical investigation, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 225 citations in OpenAlex.
- Circulating erythropoietin concentration associates with thromboembolism in sickle cell disease.British journal of haematology · 2026Article
- A minimal mathematical model of red blood cell homeostasis in anemia.PLoS computational biology · 2026Article
- Direct Generation of Immortalized Erythroid Progenitor Cell Lines from Peripheral Blood Mononuclear Cells.Cells · 2021Article
- High-Altitude Hypoxia Decreases Plasma Erythropoietin Soluble Receptor Concentration in Lowlanders.High altitude medicine & biology · 2020Article
- The localization of α-synuclein in the process of differentiation of human erythroid cells.International journal of hematology · 2018Article
- Dynamics of Erythropoietic Biomarkers in Response to Treatment With Erythropoietin in Belgrade Rats.Frontiers in pharmacology · 2018Article
- The role of spatial organization of cells in erythropoiesis.Journal of mathematical biology · 2015Article
- Clinical pharmacokinetics and pharmacodynamics of erythropoiesis-stimulating agents.Clinical pharmacokinetics · 2013Review
- A biomathematical model of human erythropoiesis under erythropoietin and chemotherapy administration.PloS one · 2013Article
- Article
- Erythropoietin activates mitochondrial biogenesis and couples red cell mass to mitochondrial mass in the heart.Circulation research · 2010Article
- Article
- Erythropoietin expands a stromal cell population that can mediate renoprotection.American journal of physiology. Renal physiology · 2008Article
- Suppression of tumor necrosis factor-induced cell death by inhibitor of apoptosis c-IAP2 is under NF-kappaB control.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Basal phosphorylation of the PEST domain in the I(kappa)B(beta) regulates its functional interaction with the c-rel proto-oncogene product.Molecular and cellular biology · 1996Article
- G-protein-coupled receptors in normal human erythroid progenitor cells.Naunyn-Schmiedeberg's archives of pharmacology · 1995Article
- Distinct roles of erythropoietin, insulin-like growth factor I, and stem cell factor in the development of erythroid progenitor cells.The Journal of clinical investigation · 1994Article
- Inhibition of human erythroid colony-forming units by tumor necrosis factor requires beta interferon.The Journal of clinical investigation · 1993Article
- Polycythemia vera blood burst-forming units-erythroid are hypersensitive to interleukin-3.The Journal of clinical investigation · 1991Article
- Inhibition of human colony-forming-unit erythroid by tumor necrosis factor requires accessory cells.The Journal of clinical investigation · 1990Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Morphological and biochemical studies of human colony-forming units-erythroid (CFU-E) have been hindered by their extreme rarity. Since burst-forming units-erythroid (BFU-E) develop into CFU-E, we used normal human blood BFU-E to generate large numbers of highly purified CFU-E in vitro. Using density centrifugation, sheep erythrocyte rosetting, surface immunoglobulin-positive cell depletion, adherence to plastic, and negative panning with monoclonal antibodies, human blood BFU-E were purified from 0.017 to 0.368%, a 22-fold purification with a 43% yield. The panned cells were cultured in methylcellulose with recombinant erythropoietin (rEp) and conditioned medium for 9 d. These cells were then collected and CFU-E were further purified using adherence and density centrifugation. This yielded almost 10(7) erythroid colony forming cells with a purity of 70 +/- 18%. Analysis of these cells by light and electron microscopy showed 94% erythroid cells. The prominent cell was a primitive blast with high nuclear/cytoplasmic ratio, dispersed nuclear chromatin and a distinct large nucleolus. The relation between the number of erythroid colonies and the number of day 9 cells plated in plasma clots was a straight line through the origin with a maximum number of erythroid colonies at 1 U/ml of rEp and no erythroid colonies without rEp. Specific binding with 125I-rEp showed that 60% of the binding was inhibited by excess pure erythropoietin (Ep), but not by albumin, fetal calf serum, and a variety of growth factors or glycoproteins. By days 12-13 of cell culture, when the progenitor cells matured to late erythroblasts, specific binding markedly declined. In this study, human CFU-E have been isolated in sufficient purity to characterize the morphology of these rare cells and in sufficient numbers to measure specific binding of Ep.
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