ArticleThe Histochemical journal1987
Histochemical procedures for the simultaneous visualization of neutral sugars and either sialic acid and its side chain O-acyl variants or O-sulphate ester. I. Methods based upon the selective periodate oxidation of sialic acids.
Article in The Histochemical journal, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 33 citations in OpenAlex.
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- Morphological and histochemical characteristics of the epithelium of ovarian lamellae of Genypterus blacodes (Schneider, 1801).Fish physiology and biochemistry · 2009Article
- Article
- How selective are the 'selective periodate oxidation' methods used in sialic acid histochemistry?The Histochemical journal · 1991Article
- Histochemical classification based upon reaction types and its application to carbohydrate histochemistry.The Histochemical journal · 1990Review
- Histochemical studies of intestinal epithelial goblet cell glycoproteins during the development of the human foetus.The Histochemical journal · 1990Article
- Application of the selective periodate oxidation of sialic acids. III. Identification of neuraminidase-sensitive and neuraminidase-resistant sialic acids and their side chain O-acyl variants.The Histochemical journal · 1988Article
- Histochemical studies of the colonic epithelial glycoproteins of the normal rabbit.The Histochemical journal · 1988Article
- Simple procedure for assessing relative quantities of neutral and acidic sugars in mucin glycoproteins: its use in assessing cyclical changes in cervical mucins.Journal of clinical pathology · 1988Article
- A new method for the histochemical demonstration of O-acyl sugars in human colonic epithelial glycoproteins.The Histochemical journal · 1988Article
- Histochemical procedures for the simultaneous visualization of neutral sugars and either sialic acid and its O-acyl variants or O-sulphate ester. II. Methods based upon the periodic acid-phenylhydrazine-Schiff reaction.The Histochemical journal · 1987Article
- A new histochemical method for the selective periodate oxidation of total tissue sialic acids.The Histochemical journalArticle
- Histochemical studies of epithelial cell glycoproteins in normal rat colon.The Histochemical journalArticle
- Methods for the identification of side chain O-acyl substituted sialic acids and for the simultaneous visualization of sialic acid, its side chain O-acyl variants and O-sulphate ester.The Histochemical journalArticle
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5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Five new methods, based upon the selective oxidation of sialic acid residues with 0.4 mM periodic acid in approximately 1 M hydrochloric acid at 4 degree C for 1 h (PA), have been devised for the simultaneous visualization of neutral sugars and either sialic acid and its side chain O-acyl variants or O-sulphate ester. In the first of these, the selective periodate oxidation-borohydride reduction-saponification-selective periodate oxidation-Thionin Schiff-saponification-borohydride reduction-periodic acid-Schiff (PA-Bh-KOH-PA-T-KOH-Bh-PAS) technique, sialic acids with O-acyl substituents at C7, C8 or C9 (or which have two of three side chain O-acyl substituents) stain blue while neutral sugars with periodate-sensitive vicinal diols (hexose, 6-deoxyhexose, and N-acetylhexosamine) stain magenta. The second method, the saponification-selective periodate oxidation-Thionin Schiff-saponification-borohydride reduction-periodic acid-Schiff (KOH-PA-T-KOH-Bh-PAS), stains all sialic acids blue and neutral sugars magenta. In the third procedure, the selective periodate oxidation-Thionin Schiff-borohydride reduction-periodic acid-Schiff-saponification (PA-T-Bh-PAS-KOH) method, sialic acids without side chain substituents (or which have an O-acyl substituent at C7) stain blue and neutral sugars stain magenta. In the fourth method, the saponification-selective periodate oxidation-borohydride reduction-Alcian Blue pH 1.0-periodic acid-Schiff (KOH-PA-Bh-AB1.0-PAS) technique, O-sulphate esters stain aquamarine blue and neutral sugars stain magenta. In all of these techniques mixtures of the components stain in various shades of purple.(ABSTRACT TRUNCATED AT 250 WORDS)
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