ArticlePlant methods2018
A protocol combining multiphoton microscopy and propidium iodide for deep 3D root meristem imaging in rice: application for the screening and identification of tissue-specific enhancer trap lines.
Article in Plant methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
7 citing papers in PubMed.
- Wheat Germ Agglutinin-Alexa Fuor488/Propidium Iodine Costaining and Fluorescence Microscopic Imaging of Ustilago maydis Infected Maize Anthers.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Label-free structural imaging of plant roots and microbes using third-harmonic generation microscopy.Scientific reports · 2025Article
- CrWRKY57 and CrABF3 cooperatively activateHorticulture research · 2025Article
- The AMSlide for noninvasive time-lapse imaging of arbuscular mycorrhizal symbiosis.Journal of microscopy · 2025Article
- Root Hair Imaging Using Confocal Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Quantitative Live Confocal Imaging inBio-protocol · 2022Article
- In situ laser manipulation of root tissues in transparent soil.Plant and soil · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe clear visualization of 3D organization at the cellular level in plant tissues is needed to fully understand plant development processes. Imaging tools allow the visualization of the main fluorophores and in vivo growth monitoring. Confocal microscopy coupled with the use of propidium iodide (PI) counter-staining is one of the most popular tools used to characterize the structure of root meristems in
resultsWe adapted a PI counter-staining protocol to visualize the internal 3D architecture of rice root meristems using multiphoton microscopy. This protocol is simple and compatible with the main fluorophores (CFP, GFP and mCherry). The efficiency and applicability of this protocol were demonstrated by screening a population of 57 enhancer trap lines. We successfully characterized GFP expression in all of the lines and identified 5 lines with tissue-specific expression.
conclusionsAll of these resources are now available for the rice community and represent critical tools for future studies of root development.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.